公开(公告)号：US06620604B1

公开(公告)日：2003-09-16

申请号：US09567003

申请日：2000-05-09

申请人： Sabine Genicot ,  Bernard Kloareg ,  Phillipe Potin ,  Brian Rudolph ,  Gerhard De Ruiter ,  Bea Penninkhof ,  Odile Richard

发明人： Sabine Genicot ,  Bernard Kloareg ,  Phillipe Potin ,  Brian Rudolph ,  Gerhard De Ruiter ,  Bea Penninkhof ,  Odile Richard

IPC分类号： C12N916

CPC分类号： C08B37/0036 ,  C08B37/0042 ,  C12N9/14

摘要： A purified sulfohydrolase having a purity level based on total amount of protein of at least about 40 wt %. Isolated nucleic acid sequence and amino acid sequences. A process for purifying at least one sulfohydrolase, including subjecting an extract from seaweed to fractionation to obtain fractions; and subjecting at least one of the fractions to phenyl sepharose chromatography to obtain sepharose fractions containing at least one sulfohydrolase. An enzymatically modified compound which has been modified by an isolated sulfohydrolase having a purity level based on total amount of protein of at least about 40 wt %. A process of enzymatically modifying a sulfated compound, including combining at least one sulfohydrolase, having a purity level based on total amount of protein of at least about 40 wt %, with a sulfated compound form a reaction mixture; and incubating the reaction mixture to remove sulfate groups from the sulfated compound to form an enzymatically modified compound. A process of enzymatically modifying a sulfated compound, including incubating a first sulfohydrolase with a sulfated compound to remove sulfate groups from the sulfated compound to form an intermediate compound; and subsequently incubating the intermediate compound with a second sulfohydrolase to remove sulfate groups to form an enzymatically modified compound. A method for extracting one of nu- and mu-carrageenan from seaweed, including dispersing seaweed in a salt solution including K2CO3 to form a dispersion; filtering the dispersion to obtain a liquid; ultrafiltering the dispersion to remove salts; concentrating the liquid; adjusting the pH of the liquid to about 8 to 8.5; and precipitating one of nu- and mu-carrageenan from the liquid.

摘要翻译： 纯度为基于蛋白质总量至少约40重量％的纯度水平的硫代水解酶。 分离的核酸序列和氨基酸序列。 一种纯化至少一种磺基水解酶的方法，包括使来自海藻的提取物进行分馏以获得级分; 并将至少一个级分进行苯基琼脂糖凝胶层析，得到含有至少一种磺基水解酶的琼脂糖凝胶馏分。 已经通过分离的磺基水解酶修饰的酶促修饰的化合物，其纯度水平基于至少约40重量％的蛋白质总量。 将硫酸化合物酶促改性的方法，包括将至少一种基于蛋白质总量至少约40重量％的纯度水平与硫酸化合物组合的磺基水解酶形成反应混合物; 并将反应混合物温育以从硫酸化合物中除去硫酸根以形成酶改性化合物。 一种酶法改性硫酸化合物的方法，包括将第一种磺基水解酶与硫酸化合物一起培养以从硫酸化合物中除去硫酸根以形成中间体化合物; 随后将中间体化合物与第二种磺基水解酶一起温育以除去硫酸根以形成酶修饰的化合物。 一种从海藻中提取一种nu-和mu-角叉菜聚糖的方法，包括将海藻分散在包含K 2 CO 3的盐溶液中形成分散体; 过滤分散液得到液体; 超滤离子以除去盐; 浓缩液体; 将液体的pH调节至约8至8.5; 并从液体中沉淀出一种nu-和mu-角叉菜胶。