公开(公告)号：US20050166290A1

公开(公告)日：2005-07-28

申请号：US11084966

申请日：2005-03-21

Applicant: Benjamin All ,  John Howard

Inventor： Benjamin All ,  John Howard

IPC: A61K39/00 ,  C07K14/14 ,  C07K14/165 ,  C12N15/40 ,  C12N15/82 ,  A61K39/02 ,  A01H1/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N15/8242 ,  C12N15/8258 ,  C12N2720/12322 ,  C12N2770/20022

Abstract: The present invention provides an expression cassette for expressing vaccine antigens in a plant cell. The expression cassette includes a DNA sequence which encodes for at least one vaccine antigen which is operably linked to transcriptional and translational control regions functional in the plant cell. The vaccine antigens of the invention are useful for protection of an animal against mucosal diseases such as Transmissible Gastroenteritis Virus (TGEV) and rotavirus. The invention also provides a transgenic plant and transgenic plant seed which has been stably transformed to express a vaccine antigen which is included in an expression cassette of the invention. The transformed plant and plant cells may be from monocot or dicot plants and include, for example, corn, soybeans, sunflower, canola or alfalfa. The transgenic plants and plant seeds of the invention may be used as a feed composition for animals. Alternatively, the transgenic plant and plant seeds of the invention may provide an immunogenic composition for protecting animals against mucosal diseases after oral administration.

Abstract translation: 本发明提供了用于在植物细胞中表达疫苗抗原的表达盒。 表达盒包括编码至少一种疫苗抗原的DNA序列，其可操作地连接到在植物细胞中功能的转录和翻译控制区。 本发明的疫苗抗原可用于保护动物免受粘膜疾病如传染性胃肠炎病毒（TGEV）和轮状病毒。 本发明还提供已经被稳定转化以表达包含在本发明的表达盒中的疫苗抗原的转基因植物和转基因植物种子。 转化的植物和植物细胞可以来自单子叶植物或双子叶植物，并且包括例如玉米，大豆，向日葵，卡诺拉或苜蓿。 本发明的转基因植物和植物种子可以用作动物的饲料组合物。 或者，本发明的转基因植物和植物种子可以提供用于在口服给药后保护动物免受粘膜疾病的免疫原性组合物。