公开(公告)号：US20070287180A1

公开(公告)日：2007-12-13

申请号：US11574484

申请日：2005-09-01

申请人： Sun-Chang Kim ,  Choong-Hoon Lee ,  Bo-Ram Lim ,  Bong-Hyun Sung ,  Byung-Jo Yu ,  Won-Sik Lee ,  Jun-Hyoung Lee ,  Sang-Hee Lee

发明人： Sun-Chang Kim ,  Choong-Hoon Lee ,  Bo-Ram Lim ,  Bong-Hyun Sung ,  Byung-Jo Yu ,  Won-Sik Lee ,  Jun-Hyoung Lee ,  Sang-Hee Lee

IPC分类号： C12N15/70 ,  C07H21/04 ,  C12N1/21

CPC分类号： C12N15/70 ,  C12N15/52

摘要： The present invention relates to Escherichia coli variants that have increased antibiotics susceptibility, diffusion efficiency, and transformation efficiency. The variants can minimize the problems caused by biofilm formation such as increased resistance to antibiotics, decreased solute diffusion efficiency, and lowered transformation efficiency. According to the present invention, when selecting genetically-modified E. coli variants, not only a lesser amount of antibiotics is required when selecting desirable variants, but also the reduction of selection efficiency caused by biofilm formation by strains other than the variants to be selected, thus decreasing exhibiting resistance to antibiotics, can be avoided. Additionally, in the process of materials production, the amount of secreted products could be increased due to the increased solute diffusion efficiency. Furthermore, the increased transformation efficiency makes the mass production of useful materials easier.

摘要翻译： 本发明涉及具有增加的抗生素敏感性，扩散效率和转化效率的大肠杆菌变体。 这些变体可以最小化由生物膜形成引起的问题，例如抗生素抗性增加，溶质扩散效率降低，转化效率降低。 根据本发明，当选择遗传修饰的大肠杆菌变体时，不仅在选择期望的变体时需要较少量的抗生素，而且还降低由选择的变体以外的菌株生物膜形成引起的选择效率 ，从而减少对抗生素的抗性。 另外，在材料生产过程中，由于溶质扩散效率的提高，分泌产物的量可能会增加。 此外，提高的转换效率使得有用材料的大量生产更容易。