公开(公告)号：US20150011482A1

公开(公告)日：2015-01-08

申请号：US14374127

申请日：2013-03-15

申请人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Rajeev Chillakuru ,  Michael Hamill

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Rajeev Chillakuru ,  Michael Hamill

IPC分类号： C07K14/435 ,  A23L1/305

CPC分类号： C07K14/435 ,  A23L33/17 ,  A23L33/18 ,  A23V2002/00 ,  A61K38/1709 ,  A23V2200/332 ,  A23V2250/54

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a first polypeptide sequence comprising a fragment of a naturally-occurring nutritive protein. In some embodiments the fragment comprises at least one of a) an enhanced ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) an enhanced ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) an enhanced ratio of essential amino acid residues to total amino acid residues present in the nutritive protein. In some embodiments, the fragment comprises at least one of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein, that is equal to or greater than the corresponding ratio present in a benchmark protein such as whey, egg or soy protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供营养蛋白质。 在一些实施方案中，营养蛋白包含包含天然存在营养蛋白片段的第一多肽序列。 在一些实施方案中，片段包含以下至少一种：a）增强的分支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例增加; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例增加。 在一些实施方案中，所述片段包含a）支链氨基酸残基与营养蛋白中存在的总氨基酸残基的比例中的至少一个; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例，其等于或大于基准蛋白如乳清，蛋或大豆蛋白中存在的相应比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。