公开(公告)号：US20060148079A1

公开(公告)日：2006-07-06

申请号：US11029541

申请日：2005-01-03

Applicant: Virgil Paunescu ,  Erika Suciu ,  Calin Tatu ,  Tudor Oprea

Inventor： Virgil Paunescu ,  Erika Suciu ,  Calin Tatu ,  Tudor Oprea

IPC: C12N5/08

CPC classification number: C12N5/0676 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/165 ,  C12N2506/1353 ,  C12N2510/02

Abstract: A method for producing an ex vivo population of human insulin-producing cells that produce insulin in response to glucose from a patient is disclosed. The population of insulin producing cells is derived from human epithelial cells by causing those epithelial cells to differentiate into insulin producing cells. In one embodiment, the epithelial cells are derived from human mesenchymal stem cells (MSCs). The MSCs can be cultured from human bone marrow. In one embodiment, a culture of epithelial cell linage is generated by cultivating a sample of human cells containing a mixed culture containing human MSCs to produce an enriched culture of MSCs having a higher concentration of MSCs than the sample; and then differentiating cells from the enriched culture into cells of the epithelial cell lineage.

Abstract translation: 公开了一种用于产生响应于来自患者的葡萄糖产生胰岛素的人胰岛素产生细胞的离体群体的方法。 通过使这些上皮细胞分化成胰岛素生成细胞，产生胰岛素的细胞群体来源于人上皮细胞。 在一个实施方案中，上皮细胞衍生自人间充质干细胞（MSC）。 MSC可以从人骨髓培养。 在一个实施方案中，通过培养含有含有人MSC的混合培养物的人细胞样品产生具有比样品更高浓度的MSC的MSCs的富集培养物来产生上皮细胞培养物的培养物; 然后将细胞从富集的培养物分化成上皮细胞谱系的细胞。