公开(公告)号：US20070072209A1

公开(公告)日：2007-03-29

申请号：US11455281

申请日：2006-06-15

申请人： Ashlee Moses ,  Klaus Frueh ,  Jeffrey King ,  James Hicks ,  Camilo Raggo ,  Jay Nelson

发明人： Ashlee Moses ,  Klaus Frueh ,  Jeffrey King ,  James Hicks ,  Camilo Raggo ,  Jay Nelson

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/5011 ,  G01N33/57407

摘要： Aspects of the present invention use gene expression profiling, and gene silencing methods to identify and provide a plurality of ‘validated’ KSHV-induced cellular gene sequences and pathways useful as targets for modulation of KSHV-mediated effects on cellular proliferation and phenotype (e.g., cancer) associated with latent and lytic phases of the Kaposi's sarcoma-associated herpesvirus (KSHV; Human herpesvirus 8; HHV8) life cycle. Particular embodiments provide therapeutic compositions, and methods for modulation and treatment of KSHV infection or KSHV-mediated effects on cellular proliferation and phenotype, comprising inhibition of KSHV-induced gene sequences or products thereof. Additional embodiments provide screening assays for compounds useful to modulate KSHV infection or KSHV-mediated effects on cellular proliferation and phenotype. Further embodiments provide diagnostic and/or prognostic assays for KSHV infection or related conditions. Additional embodiments provide novel in vivo models for KSHV infection or related conditions. Yet further aspects provide novel methods for transforming a mammalian cell, comprising expressing, by recombinant means, a transforming amount of RDCI, Neuritin, or both, and further provide cells transformed thereby.

摘要翻译： 本发明的方面使用基因表达谱和基因沉默方法来鉴定并提供多个“验证的”KSHV诱导的细胞基因序列和可用作调节KSHV介导的细胞增殖和表型效应的靶标的途径（例如， 癌症）与卡波西肉瘤相关疱疹病毒（KSHV;人类疱疹病毒8; HHV8）生命周期的潜伏和溶解期相关。 具体实施方案提供用于调节和治疗KSHV感染或KSHV介导的对细胞增殖和表型的影响的治疗组合物和方法，包括KSHV诱导的基因序列或其产物的抑制。 另外的实施方案提供了用于调节KSHV感染或KSHV介导的细胞增殖和表型效应的化合物的筛选测定。 另外的实施方案提供用于KSHV感染或相关病症的诊断和/或预后测定。 另外的实施方案提供用于KSHV感染或相关条件的新的体内模型。 另外的方面提供了用于转化哺乳动物细胞的新方法，包括通过重组方式表达转化量的RDCI，神经细胞或二者，并进一步提供由其转化的细胞。

公开(公告)号：US20060275769A1

公开(公告)日：2006-12-07

申请号：US10541598

申请日：2004-01-06

申请人： Ashlee Moses ,  Klaus Fruh ,  Jeffrey King ,  James Hicks ,  Camilo Raggo ,  Jay Nelson

发明人： Ashlee Moses ,  Klaus Fruh ,  Jeffrey King ,  James Hicks ,  Camilo Raggo ,  Jay Nelson

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： A01K67/0271 ,  A01K2227/105 ,  A01K2267/0331 ,  A01K2267/0337 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2320/12 ,  C12Q1/6886 ,  C12Q1/705 ,  C12Q2600/136 ,  C12Q2600/158

摘要： The present invention uses gene expression profiling, and gene silencing methods to identify and provide a plurality of ‘validated’ KSHV-induced cellular gene sequences and pathways useful as targets for modulation of KSHV-mediated effects on cellular proliferation and phenotype (e.g., cancer) associated with latent and lytic phases of the Kaposi's sarcoma-associated herpesvirus (KSHV; Human herpesvirus 8; HHV8) life cycle. Particular embodiments provide therapeutic compositions, and methods for modulation of KSHV infection or KSHV-mediated effects on cellular proliferation and phenotype, comprising inhibition of KSHV-induced gene sequences. Additional embodiments provide screening assays for compounds useful to modulate KSHV infection or KSHV-mediated effects on cellular proliferation and phenotype. Further embodiments provide diagnostic and/or prognostic assays for KSHV infection.

摘要翻译： 本发明使用基因表达谱和基因沉默方法来鉴定并提供多个“验证的”KSHV诱导的细胞基因序列和途径，其可用作调节KSHV介导的对细胞增殖和表型（例如癌症）的作用的靶标， 与卡波西肉瘤相关疱疹病毒（KSHV;人类疱疹病毒8; HHV8）生命周期的潜伏和溶解期相关。 具体实施方案提供用于调节KSHV感染或KSHV介导的对细胞增殖和表型的影响的治疗组合物和方法，包括KSHV诱导的基因序列的抑制。 另外的实施方案提供了用于调节KSHV感染或KSHV介导的细胞增殖和表型效应的化合物的筛选测定。 其他实施方案提供用于KSHV感染的诊断和/或预后测定。