公开(公告)号：US09322055B2

公开(公告)日：2016-04-26

申请号：US14009304

申请日：2012-03-30

申请人： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael Pallas ,  Marcin Sikora ,  Casey McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

发明人： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael Pallas ,  Marcin Sikora ,  Casey McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N21/6486 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/159 ,  C12Q2565/626

摘要： Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

摘要翻译： 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。

公开(公告)号：US20140248623A1

公开(公告)日：2014-09-04

申请号：US14009304

申请日：2012-03-30

申请人： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael Pallas ,  Marcin Sikora ,  Casey McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

发明人： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael Pallas ,  Marcin Sikora ,  Casey McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N21/6486 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/159 ,  C12Q2565/626

摘要： Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

摘要翻译： 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。