公开(公告)号：US07995205B2

公开(公告)日：2011-08-09

申请号：US12273299

申请日：2008-11-18

申请人： Peter Miller ,  David Fletcher-Holmes ,  Cathy M. Boutin ,  Clifford Hoyt

发明人： Peter Miller ,  David Fletcher-Holmes ,  Cathy M. Boutin ,  Clifford Hoyt

IPC分类号： G01J4/00

CPC分类号： G01J4/04 ,  G01N21/23

摘要： Apparatus and methods are disclosed for viewing low-birefringence structures within samples directly, with the eye, in real-time. The sample is placed between an entrance polarizer and analyzer polarizer, the transmission state of one of which is changed dynamically to create a modulated view of the scene; against this background, birefringent structures are visible because of their different appearance when modulated. Modulation rates of 4 or more states per second; use of 4 or more states, or even a continuum of states, which lie substantially on a latitude line on the Poincare sphere; and orientation of the polarization components to produce a uniform background; produce a clear view that does not produce operator fatigue. Broad-band wavelength operation spanning 50 nm or more, or the whole visible range, is achieved, and it is compatible with integration into other microscopy modes such as Hoffman relief contrast.

摘要翻译： 公开了用于在眼睛内实时观察样品内的低双折射结构的装置和方法。 将样品放置在入射偏振器和分析器偏振器之间，其中一个的传输状态被动态地改变以产生场景的调制视图; 在这种背景下，双折射结构是可见的，因为它们在调制时的外观不同。 每秒4个或更多个状态的调制率; 使用4个或更多的状态，甚至是连续的状态，它们基本上位于庞加莱球体上的纬度线上; 和偏振分量的取向产生均匀的背景; 产生不会产生操作员疲劳的清晰视图。 实现跨越50nm或更大，或整个可见光范围的宽带波长操作，并且与其他显微镜模式（例如Hoffman浮雕对比度）的集成兼容。

公开(公告)号：US20090135422A1

公开(公告)日：2009-05-28

申请号：US12273299

申请日：2008-11-18

申请人： Peter Miller ,  David Fletcher-Holmes ,  Cathy M. Boutin ,  Clifford Hoyt

发明人： Peter Miller ,  David Fletcher-Holmes ,  Cathy M. Boutin ,  Clifford Hoyt

IPC分类号： G01J4/00

CPC分类号： G01J4/04 ,  G01N21/23

摘要： Apparatus and methods are disclosed for viewing low-birefringence structures within samples directly, with the eye, in real-time. The sample is placed between an entrance polarizer and analyzer polarizer, the transmission state of one of which is changed dynamically to create a modulated view of the scene; against this background, birefringent structures are visible because of their different appearance when modulated. Modulation rates of 4 or more states per second; use of 4 or more states, or even a continuum of states, which lie substantially on a latitude line on the Poincare sphere; and orientation of the polarization components to produce a uniform background; produce a clear view that does not produce operator fatigue. Broad-band wavelength operation spanning 50 nm or more, or the whole visible range, is achieved, and it is compatible with integration into other microscopy modes such as Hoffman relief contrast.

摘要翻译： 公开了用于在眼睛内实时观察样品内的低双折射结构的装置和方法。 将样品放置在入射偏振器和分析器偏振器之间，其中一个的传输状态被动态地改变以产生场景的调制视图; 在这种背景下，双折射结构是可见的，因为它们在调制时的外观不同。 每秒4个或更多个状态的调制率; 使用4个或更多的状态，甚至是连续的状态，它们基本上位于庞加莱球体上的纬度线上; 和偏振分量的取向产生均匀的背景; 产生不会产生操作员疲劳的清晰视图。 实现跨越50nm或更大，或整个可见光范围的宽带波长操作，并且与其他显微镜模式（例如Hoffman浮雕对比度）的集成兼容。