Methods for surface modification of silica for use in capillary zone electrophoresis and chromatography
    1.
    发明授权
    Methods for surface modification of silica for use in capillary zone electrophoresis and chromatography 失效
    用于毛细管区带电泳和色谱法的二氧化硅表面改性方法

    公开(公告)号:US06776911B2

    公开(公告)日:2004-08-17

    申请号:US10069567

    申请日:2002-07-03

    IPC分类号: B01D1508

    CPC分类号: G01N27/44752

    摘要: The present invention refers to the use of novel molecules able to bind tenaciously to silica, borosilicate and silicate surfaces, and thus to modify their properties and characteristics. When applied to silica-based chromatography, it offers important advantages in all cases in which it is necessary to modulate the interaction of analytes with the stationary phase. In capillary zone electrophoresis (CZE), such compounds will eliminate or invert the electroendoosmostic (EEO) flow, greatly simplifying the analysis of negatively-charged compounds and permitting the analysis of bio(macro)molecules via the direct use of naked capillaries.

    摘要翻译: 本发明涉及能够牢固地结合二氧化硅,硼硅酸盐和硅酸盐表面,从而改变其性质和特性的新型分子的用途。 当应用于二氧化硅基色谱时,它在所有需要调节分析物与固定相的相互作用的情况下提供重要的优点。 在毛细管区带电泳(CZE)中,这种化合物将消除或反转电活性(EEO)流,大大简化了带负电荷的化合物的分析,并允许通过直接使用裸露毛细管分析生物(宏观)分子。