公开(公告)号：US08528111B2

公开(公告)日：2013-09-03

申请号：US13176689

申请日：2011-07-05

申请人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

发明人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

IPC分类号： G01Q30/02

CPC分类号： G01Q60/42 ,  G01Q30/025

摘要： A method for positioning a tip of an atomic force microscope relative to a intracellular target site in a cell is provided. In general terms, the method comprises: a) positioning a fluorescent tip of an atomic force microscope over a cell comprising a fluorescent intracellular target site so that said tip is above target site; b) moving the tip toward said target site while obtaining images of the distal end of said tip and/or the target site using a fluorescence microscope; and c) arresting the movement of the tip when the target site and the distal end of the tip are both in focus in the fluorescence microscope. A microscope system for performing the method is also provided.

摘要翻译： 提供了一种用于将原子力显微镜的尖端相对于细胞中的细胞内靶位点定位的方法。 通常来说，该方法包括：a）将原子力显微镜的荧光尖端定位在包含荧光细胞内靶位点的细胞上，使得所述尖端高于目标位点; b）使用荧光显微镜获得所述尖端和/或靶位点的远端的图像，使尖端移向所述目标部位; 以及c）当荧光显微镜中的尖端的目标部位和远端都聚焦时，阻止尖端的运动。 还提供了用于执行该方法的显微镜系统。

公开(公告)号：US20130014295A1

公开(公告)日：2013-01-10

申请号：US13176689

申请日：2011-07-05

申请人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

发明人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

IPC分类号： G01Q10/00

CPC分类号： G01Q60/42 ,  G01Q30/025

摘要： A method for positioning a tip of an atomic force microscope relative to a intracellular target site in a cell is provided. In general terms, the method comprises: a) positioning a fluorescent tip of an atomic force microscope over a cell comprising a fluorescent intracellular target site so that said tip is above target site; b) moving the tip toward said target site while obtaining images of the distal end of said tip and/or the target site using a fluorescence microscope; and c) arresting the movement of the tip when the target site and the distal end of the tip are both in focus in the fluorescence microscope. A microscope system for performing the method is also provided.

摘要翻译： 提供了一种用于将原子力显微镜的尖端相对于细胞中的细胞内靶位点定位的方法。 通常来说，该方法包括：a）将原子力显微镜的荧光尖端定位在包含荧光细胞内靶位点的细胞上，使得所述尖端高于目标位点; b）使用荧光显微镜获得所述尖端和/或靶位点的远端的图像，使尖端移向所述目标部位; 以及c）当荧光显微镜中的尖端的目标部位和远端都聚焦时，阻止尖端的运动。 还提供了用于执行该方法的显微镜系统。