公开(公告)号：US07189808B2

公开(公告)日：2007-03-13

申请号：US10152212

申请日：2002-05-20

申请人： Johannes Gerdes ,  Thomas Scholzen ,  Claudia Wohlenberg

发明人： Johannes Gerdes ,  Thomas Scholzen ,  Claudia Wohlenberg

IPC分类号： C07K14/435 ,  A61K38/17

CPC分类号： C12N15/87 ,  A61K38/00 ,  C07K14/4738 ,  C07K2319/00

摘要： The invention relates to the use of a carboxy-terminal fragment of the Ki-67 protein or of an active part, fragment or homologue thereof as a compound that can be used for intracellular transfer and for the introduction in and the release by the cells. The invention further relates to transfer compounds that contain the above-mentioned Ki-67 protein and to the vectors encoding the same. The invention also relates to corresponding pharmaceutical compositions and to the use of the transfer protein as an excipient or active agent in the treatment of diseases.

摘要翻译： 本发明涉及Ki-67蛋白或其活性部分，其片段或同源物的羧基末端片段作为可用于细胞内转移和引入细胞释放的化合物的用途。 本发明还涉及含有上述Ki-67蛋白质的转移化合物及其编码载体。 本发明还涉及相应的药物组合物以及转运蛋白作为赋形剂或活性剂在治疗疾病中的用途。

公开(公告)号：US06777194B1

公开(公告)日：2004-08-17

申请号：US09937649

申请日：2002-01-28

申请人： Johannes Gerdes ,  Thomas Scholzen ,  Elmar Endi ,  Claudia Wohlenberg ,  Bettina Baron-Lühr ,  Margrit Kernbach Hahn ,  Patricia Prilla ,  Johanna Suwinski ,  Rolf Knippers

发明人： Johannes Gerdes ,  Thomas Scholzen ,  Elmar Endi ,  Claudia Wohlenberg ,  Bettina Baron-Lühr ,  Margrit Kernbach Hahn ,  Patricia Prilla ,  Johanna Suwinski ,  Rolf Knippers

IPC分类号： G01N33574

CPC分类号： C07K16/14 ,  A61K2039/505 ,  Y10S435/975

摘要： The present invention relates to monoclonal antibodies against the human Mcm3 protein, hybridoma cell lines that produce such antibodies, procedures for the production and their use, pharmaceutical compositions comprising a monoclonal antibody according to the present invention, their use for the prevention and treating of certain diseases as well as methods relating to the prevention and treatment of diseases associated with Mcm3 expression. Monoclonal antibodies according to the invention detect and bind human Mcm3 monospecifically both in immunohistological and immunobiochemical detection systems. The process for the production of these monoclonal antibodies comprises an initial screening of excess hybridoma supernatant with immunobiochemical methods followed by a second screening of positive hybridoma by means of an immunohistochemical method.

摘要翻译： 本发明涉及针对人Mcm3蛋白的单克隆抗体，产生此类抗体的杂交瘤细胞系，其制备方法及其用途，包含本发明单克隆抗体的药物组合物，其用于预防和治疗某些 疾病以及与预防和治疗与Mcm3相关的疾病相关的方法。 根据本发明的单克隆抗体在免疫组织化学和免疫生物化学检测系统中单独检测并结合人Mcm3。 制备这些单克隆抗体的方法包括用免疫生物化学方法初步筛选过量的杂交瘤上清液，然后通过免疫组织化学方法对阳性杂交瘤进行第二次筛选。