公开(公告)号：US20180362504A1

公开(公告)日：2018-12-20

申请号：US16062423

申请日：2016-12-15

Applicant: Joseph FOX ,  Xinqiao JIA ,  Will TROUT ,  Joel ROSENTHAL ,  Han ZHANG ,  Yinzhi FANG ,  Colin THORPE ,  Shuang LIU ,  Yixin XIE

Inventor： Joseph FOX ,  Xinqiao JIA ,  Will TROUT ,  Joel ROSENTHAL ,  Han ZHANG ,  Yinzhi FANG ,  Colin THORPE ,  Shuang LIU ,  Yixin XIE

IPC: C07D401/14 ,  C07D401/04 ,  C12P17/16

Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein one R group on the dihydrotetrazine 1 is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the other R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl,—or heteroatom-containing groups; 1, 2 wherein the method comprises oxidizing dihydrotetrazine 1 in a reaction medium in the presence of a catalyst and a stoichiometric oxidant.

公开(公告)号：US07625733B2

公开(公告)日：2009-12-01

申请号：US12220464

申请日：2008-07-24

Applicant: Colin Thorpe ,  Jennifer Jaje

Inventor： Colin Thorpe ,  Jennifer Jaje

IPC: C12N9/02 ,  A23J1/00

CPC classification number: C12N9/0051

Abstract: Methods for isolating a highly purified QSOX enzyme from milk or whey are described. The enzyme may be used to generate intermolecular and intramolecular disulfide bridges and to facilitate oxidative protein folding.

Abstract translation: 描述了从牛奶或乳清分离高纯度QSOX酶的方法。 该酶可用于产生分子间和分子内二硫键并促进氧化蛋白质折叠。

公开(公告)号：US11485987B2

公开(公告)日：2022-11-01

申请号：US17132532

申请日：2020-12-23

Applicant: Joseph M. Fox ,  Colin Thorpe ,  Amanda M. Tallon ,  Yixin Xie ,  William Trout ,  Ashlyn S. Cantrel ,  Julia E. Rosenberger

Inventor： Joseph M. Fox ,  Colin Thorpe ,  Amanda M. Tallon ,  Yixin Xie ,  William Trout ,  Ashlyn S. Cantrel ,  Julia E. Rosenberger

IPC: C12P17/12 ,  C12P17/16

Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein the dihydrotetrazine 1 comprises a first R group and a second R group, wherein the first R group is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the second R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, and heteroatom-containing groups; wherein the method comprises (a) providing the dihydrotetrazine 1 in a reaction medium, and (b) adding an enzyme as a catalyst and an oxidant to the reaction medium, whereby the dihydrotetrazine 1 is converted to the tetrazine 2.

公开(公告)号：US20210164005A1

公开(公告)日：2021-06-03

申请号：US17132532

申请日：2020-12-23

Applicant: Joseph M. FOX ,  Colin THORPE ,  Amanda M. TALLON ,  Yixin XIE ,  William TROUT ,  Ashlyn S. CANTREL ,  Julia E. ROSENBERGER

Inventor： Joseph M. FOX ,  Colin THORPE ,  Amanda M. TALLON ,  Yixin XIE ,  William TROUT ,  Ashlyn S. CANTREL ,  Julia E. ROSENBERGER

IPC: C12P17/16 ,  C12P17/12

Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein the dihydrotetrazine 1 comprises a first R group and a second R group, wherein the first R group is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the second R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, and heteroatom-containing groups; wherein the method comprises (a) providing the dihydrotetrazine 1 in a reaction medium, and (b) adding an enzyme as a catalyst and an oxidant to the reaction medium, whereby the dihydrotetrazine 1 is converted to the tetrazine 2.

公开(公告)号：US07045547B2

公开(公告)日：2006-05-16

申请号：US10223385

申请日：2002-08-20

Applicant: Colin Thorpe ,  Wenzhong Wang

Inventor： Colin Thorpe ,  Wenzhong Wang

IPC: A61K31/21 ,  A61K31/33 ,  C07C327/00

CPC classification number: C07C327/30 ,  C07H19/20

Abstract: Compounds of formula I wherein A is R3, OR3, SR3, and NR3R4; and R1, R2, R3 and R4 are as defined. The compounds of the invention are reversible inhibitors of acyl-CoA dehydrogenase, and are useful in treating disorders such as diabetes, heart diseases (including angina and congestive heart failure), and peripheral vascular disease in patients suffering from these diseases or conditions resulting thereof.

Abstract translation: 其中A为R 3，OR 3，SR 3和NR 3的式I化合物 > 4 ; 且R 1，R 2，R 3和R 4如上所定义。 本发明的化合物是酰基辅酶A脱氢酶的可逆抑制剂，并且可用于治疗患有这些疾病或病症的患者中的疾病如糖尿病，心脏病（包括心绞痛和充血性心力衰竭）和外周血管疾病。

公开(公告)号：US20090042269A1

公开(公告)日：2009-02-12

申请号：US12220464

申请日：2008-07-24

Applicant: Colin Thorpe ,  Jennifer Jaje

Inventor： Colin Thorpe ,  Jennifer Jaje

IPC: C12N9/02

CPC classification number: C12N9/0051

Abstract: Methods for isolating a highly purified QSOX enzyme from milk or whey are described. The enzyme may be used to generate intermolecular and intramolecular disulfide bridges and to facilitate oxidative protein folding.