Method for recovering plant-derived proteins

    公开(公告)号:US09815873B2

    公开(公告)日:2017-11-14

    申请号:US14006552

    申请日:2012-03-23

    IPC分类号: C07K14/415 C07K1/14

    CPC分类号: C07K14/415 C07K1/145

    摘要: Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.

    METHOD OF PREPARING PLANT-DERIVED PROTEINS
    2.
    发明申请
    METHOD OF PREPARING PLANT-DERIVED PROTEINS 审中-公开
    制备植物衍生蛋白的方法

    公开(公告)号:US20130067807A1

    公开(公告)日:2013-03-21

    申请号:US13497767

    申请日:2010-09-21

    摘要: Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.

    摘要翻译: 提供了制备植物衍生蛋白或超基质蛋白的方法。 该方法可以包括获得植物或包含质外体蛋白质定位蛋白质的植物物质或超基质蛋白,从植物或植物物质产生原生质体/原生质体级分和质外体级分,并回收载脂蛋白级分。 载脂蛋白级分包含植物来源的蛋白质或超基因蛋白质。 或者,可以通过使用细胞壁降解酶组合物消化植物物质以产生消化部分,从包含植物衍生的蛋白质或超基质蛋白质的植物或植物物质获得蛋白质或超基质蛋白质。 将消化的馏分过滤以产生过滤的馏分,从过滤的馏分中回收植物来源的蛋白质或超结构蛋白质。

    METHOD FOR RECOVERING PLANT-DERIVED PROTEINS
    3.
    发明申请
    METHOD FOR RECOVERING PLANT-DERIVED PROTEINS 有权
    恢复植物衍生蛋白的方法

    公开(公告)号:US20140024104A1

    公开(公告)日:2014-01-23

    申请号:US14006552

    申请日:2012-03-23

    IPC分类号: C07K14/415

    CPC分类号: C07K14/415 C07K1/145

    摘要: Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.

    摘要翻译: 提供了回收植物衍生蛋白或超基质蛋白的方法。 该方法可以包括获得植物或包含质外质蛋白定位的蛋白质的植物物质或超基因蛋白质,松动细胞壁以产生具有松散的细胞壁的植物或植物物质,从而允许通过细胞壁将载脂蛋白含量释放到 从植物或植物物质中产生载脂蛋白含量,并回收载脂蛋白含量。 载脂蛋白含量分数包括植物来源的蛋白质或超基因蛋白质。

    METHOD OF PREPARING PLANT-DERIVED VLPS
    4.
    发明申请
    METHOD OF PREPARING PLANT-DERIVED VLPS 审中-公开
    制备植物衍生VLPS的方法

    公开(公告)号:US20120178149A1

    公开(公告)日:2012-07-12

    申请号:US13497757

    申请日:2010-09-21

    IPC分类号: C12S3/14

    摘要: Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.

    摘要翻译: 提供了植物来源的病毒样颗粒(VLPs)的制备方法。 该方法可以包括获得植物或包含原生质体定位的VLP的植物物质,从植物或植物物质产生原生质体/原生质体级分和原生质体级分,以及回收载脂蛋白级分。 载脂蛋白级分包含植物来源的VLP。 或者,VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得,以产生消化部分。 将消化的馏分过滤以产生过滤的馏分,从过滤的馏分中回收来自植物的VLP。