公开(公告)号：US07663026B2

公开(公告)日：2010-02-16

申请号：US10546558

申请日：2003-07-07

Applicant: Song Qin ,  Peng Jiang ,  Daozhan Yu ,  Fuchao Li ,  Guoqiong Sun

Inventor： Song Qin ,  Peng Jiang ,  Daozhan Yu ,  Fuchao Li ,  Guoqiong Sun

IPC: C12N15/82

CPC classification number: C12N15/8242 ,  C12N15/8207

Abstract: This invention relates to marine algae, and more particularly, to a method for producing improved seaweed strains by genetic engineering. The vector for transformation were constructed by inserting the high-plant or algae-derived promoters upstream of foreign reporter genes or such cassettes that functional genes are fused with antibiotics or herbicide-resistant genes. The genetic seaweed was generated by natural development process by recombinated plasmid DNA introduction to seaweed spore with Biolostics as transformation methods. Introduced traits of antibiotics or herbicide-resistance were used to select the transgenic seaweed individuals when foreign functional genes are transformed. Stable transformation could be obtained following this invention.

Abstract translation: 本发明涉及海藻，更具体地说，涉及通过遗传工程生产改良的海藻菌株的方法。 通过将外源报告基因上游的高植物或藻类来源的启动子或功能基因与抗生素或除草剂抗性基因融合的盒子插入，构建转化载体。 通过重组质粒DNA引入海藻孢子以Biolostics作为转化方法，通过自然发育过程产生遗传海藻。 当外源功能基因转化时，引入抗生素或除草剂抗性的特征用于选择转基因海草个体。 本发明可以获得稳定的转化。

公开(公告)号：US20060154355A1

公开(公告)日：2006-07-13

申请号：US10546558

申请日：2003-07-07

Applicant: Song Qin ,  Peng Jiang ,  Daozhan Yu ,  Fuchao Li ,  Guoqiong Sun

Inventor： Song Qin ,  Peng Jiang ,  Daozhan Yu ,  Fuchao Li ,  Guoqiong Sun

IPC: C12N1/12 ,  C12N15/74

CPC classification number: C12N15/8242 ,  C12N15/8207

Abstract: This invention relates to marine algae, and more particularly, to a method for producing improved seaweed strains by genetic engineering. The vector for transformation were constructed by inserting the high-plant or algae-derived promoters upstream of foreign reporter genes or such cassettes that functional genes are fused with antibiotics or herbicide-resistant genes. The genetic seaweed was generated by natural development process by recombinated plasmid DNA introduction to seaweed spore with Biolostics as transformation methods. Introduced traits of antibiotics or herbicide-resistance were used to select the transgenic seaweed individuals when foreign functional genes are transformed. Stable transformation could be obtained following this invention.

Abstract translation: 本发明涉及海藻，更具体地说，涉及通过遗传工程生产改良的海藻菌株的方法。 通过将外源报告基因上游的高植物或藻类来源的启动子或功能基因与抗生素或除草剂抗性基因融合的盒子插入，构建转化载体。 通过重组质粒DNA引入海藻孢子以Biolostics作为转化方法，通过自然发育过程产生遗传海藻。 当外源功能基因转化时，引入抗生素或除草剂抗性的特征用于选择转基因海草个体。 本发明可以获得稳定的转化。