公开(公告)号：US20080318325A1

公开(公告)日：2008-12-25

申请号：US11922366

申请日：2006-06-28

申请人： Gabriel Blume ,  Michael Sacher ,  Dirk Teichmueller ,  Katinka Jung

发明人： Gabriel Blume ,  Michael Sacher ,  Dirk Teichmueller ,  Katinka Jung

IPC分类号： G01N33/92 ,  G01N24/00

CPC分类号： G01N24/10 ,  Y10T436/24

摘要： A method for the determination of the morphological integrity of a membrane of lipid vesicles, such as liposomes, using electron spin resonance (ESR) spectroscopy including the steps of: a) labeling the lipid vesicles with an ESR-active probe; b) producing a sample by introducing a quantity of the labeled lipid vesicles into a test medium; c) producing a positive control by introducing a quantity of the labeled lipid vesicles into a control medium and optionally a negative control by introducing a quantity of the ESR-active probe into the test medium; d) obtaining ESR spectra of the controls and the sample; and e) comparing ESR spectra of the sample and controls to determine relative morphological integrity. Morphological integrity of lipid vesicles may be quantitatively determined in the test medium by obtaining difference spectra produced using the spectra of the sample and of the positive and/or negative control.

摘要翻译： 一种使用电子自旋共振（ESR）光谱法测定脂质囊泡膜脂质囊膜形态完整性的方法，包括以下步骤：a）用ESR活性探针标记脂质囊泡; b）通过将一定量的标记的脂质囊泡引入到测试介质中来生产样品; c）通过将一定量的ESR活性探针引入测试培养基中，将一定数量的标记的脂质囊泡引入对照培养基和任选的阴性对照中来产生阳性对照; d）获得对照和样品的ESR光谱; 和e）比较样品和对照的ESR光谱以确定相对形态学完整性。 脂质囊泡的形态完整性可以通过获得使用样品和阳性和/或阴性对照的光谱产生的差异光谱在测试培养基中定量测定。