公开(公告)号：US12031941B2

公开(公告)日：2024-07-09

申请号：US16992054

申请日：2020-08-12

申请人： ERBI BIOSYSTEMS, INC.

发明人： Harry Lee ,  Kevin Shao-Kwan Lee

IPC分类号： G01N27/416 ,  G01N33/00 ,  G05D21/02 ,  G01D18/00

CPC分类号： G01N27/4165 ,  G01N33/004 ,  G05D21/02 ,  G01D18/008

摘要： Methods of calibrating a pH sensor fixed within an enclosed vessel are disclosed. The methods include introducing a buffer into the enclosed vessel, introducing a gas mixture comprising CO2 into the enclosed vessel, measuring a pH signal of the solution, measuring a CO2 concentration of a headspace gas of the solution, and calculating a pH value with a buffer calibration curve. The methods include calculating a calibration parameter with a sensor calibration curve and calibrating the pH sensor with the calibration parameter. Reactor systems are also disclosed. The systems include an enclosed reactor, a pH sensor, a CO2 sensor, a temperature control subsystem, and a controller. Methods of facilitating pH sensor calibration without sampling in a bioreactor system are also disclosed. The methods include providing a controller and providing instructions to operably connect the controller to the pH sensor and the CO2 sensor.

公开(公告)号：US20210048408A1

公开(公告)日：2021-02-18

申请号：US16992054

申请日：2020-08-12

申请人： ERBI BIOSYSTEMS, INC.

发明人： Harry Lee ,  Kevin Shao-Kwan Lee

IPC分类号： G01N27/416 ,  G05D21/02 ,  G01N33/00

摘要： Methods of calibrating a pH sensor fixed within an enclosed vessel are disclosed. The methods include introducing a buffer into the enclosed vessel, introducing a gas mixture comprising CO2 into the enclosed vessel, measuring a pH signal of the solution, measuring a CO2 concentration of a headspace gas of the solution, and calculating a pH value with a buffer calibration curve. The methods include calculating a calibration parameter with a sensor calibration curve and calibrating the pH sensor with the calibration parameter. Reactor systems are also disclosed. The systems include an enclosed reactor, a pH sensor, a CO2 sensor, a temperature control subsystem, and a controller. Methods of facilitating pH sensor calibration without sampling in a bioreactor system are also disclosed. The methods include providing a controller and providing instructions to operably connect the controller to the pH sensor and the CO2 sensor.