公开(公告)号：US20170314070A1

公开(公告)日：2017-11-02

申请号：US15522812

申请日：2015-10-30

Applicant: EWHA UNIVERSITY - UNDUSTRY COLLBARATION FOUNDATION ,  KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyukjin LEE ,  Haeshin LEE ,  Ho Yeon LEE ,  Il Young JUNG

IPC: C12Q1/68 ,  B01L3/00

Abstract: The present invention provides facile and accurate molecular diagnosis of disease-specific genes capable of the naked eye detection through amplifying the target genes to selectively block the fluid path in a microfluidic device. Specifically, the present invention includes an isothermal amplification of target genes through a rolling circle amplification, a microfluidic device for detecting pathogen genes, and a detection method using the same. Therefore, the present invention can conveniently detect a single target gene, such as a single pathogen, or at the same time, several target genes, such as several pathogens, without complicated mechanical equipment.