公开(公告)号：US07881517B2

公开(公告)日：2011-02-01

申请号：US11611934

申请日：2006-12-18

申请人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

发明人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  G06T7/0012

摘要： A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

摘要翻译： 提供了一种用于比较包含多个组分的细胞提取物的多个样品的方法。 该方法包括以下步骤：从至少两组细胞制备细胞提取物的至少两个样品，并将每个所述细胞提取物的所述样品暴露于一组匹配的标记物，例如， 将标记物结合到细胞提取物以标记细胞提取物，所述标记物组内的每个标记物能够结合细胞提取物，并且可以从所述组中的所有其它标记物单独检测。 然后将样品混合以形成混合物，并将所述混合物电泳以分离细胞提取物内的组分。 通过检测各个标记获得电泳混合物的至少两个电子图像（I），每个图像通过检测与其他标记不同的标记来表示。 创建获得的至少两个电子图像的一个结果电子图像（Ires）（II）并进行分析以识别点分析区域（III）。 所识别的斑点分析区域被应用于相应的至少两个电子图像，用于评估所述区域，以便检测表示所述细胞提取物（IV）的组分的斑点。

公开(公告)号：US20070292044A1

公开(公告)日：2007-12-20

申请号：US11611934

申请日：2006-12-18

申请人： ERIK SLOGE ,  TOBIAS SODERMAN ,  LENNART BJORKESTEN

发明人： ERIK SLOGE ,  TOBIAS SODERMAN ,  LENNART BJORKESTEN

IPC分类号： G06K9/40

CPC分类号： G06K9/00127 ,  G06T7/0012

摘要： A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

摘要翻译： 提供了一种用于比较包含多个组分的细胞提取物的多个样品的方法。 该方法包括以下步骤：从至少两组细胞制备细胞提取物的至少两个样品，并将每个所述细胞提取物的所述样品暴露于一组匹配的标记物，例如， 将标记物结合到细胞提取物以标记细胞提取物，所述标记物组内的每个标记物能够结合细胞提取物，并且可以从所述组中的所有其它标记物单独检测。 然后将样品混合以形成混合物，并将所述混合物电泳以分离细胞提取物内的组分。 通过检测各个标记获得电泳混合物的至少两个电子图像（I），每个图像通过检测与其他标记不同的标记来表示。 创建（II）获得的至少两个电子图像的一个结果电子图像（I），并分析以识别点分析区域（III）。 所识别的斑点分析区域被应用于相应的至少两个电子图像，用于评估所述区域，以便检测表示所述细胞提取物（IV）的组分的斑点。

公开(公告)号：US07155050B1

公开(公告)日：2006-12-26

申请号：US10129448

申请日：2000-10-31

申请人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

发明人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  G06T7/0012

摘要： A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

摘要翻译： 提供了一种用于比较包含多个组分的细胞提取物的多个样品的方法。 该方法包括以下步骤：从至少两组细胞制备细胞提取物的至少两个样品，并将每个所述细胞提取物的所述样品暴露于一组匹配的标记物，例如， 将标记物结合到细胞提取物以标记细胞提取物，所述标记物组内的每个标记物能够结合细胞提取物，并且可以从所述组中的所有其它标记物单独检测。 然后将样品混合以形成混合物，并将所述混合物电泳以分离细胞提取物内的组分。 通过检测各个标记获得电泳混合物的至少两个电子图像（I），每个图像通过检测与其他标记不同的标记来表示。 创建（II）获得的至少两个电子图像的一个结果电子图像（I），并分析以识别点分析区域（III）。 所识别的斑点分析区域被应用于相应的至少两个电子图像，用于评估所述区域，以便检测表示所述细胞提取物（IV）的组分的斑点。