公开(公告)号：US20060211006A1

公开(公告)日：2006-09-21

申请号：US11183499

申请日：2005-07-18

申请人： Flavio Schenkel ,  Stephen Miller ,  Zhihua Jiang

发明人： Flavio Schenkel ,  Stephen Miller ,  Zhihua Jiang

IPC分类号： C12Q1/68 ,  G06F19/00 ,  C07H21/04

CPC分类号： C12Q1/6888 ,  C12Q2600/156

摘要： The present invention relates to the identification of a single nucleotide polymorphism (SNP) within the bovine CAST locus encoding the calpastatin protein, wherein the allelic variation of the SNP is a G/C transversion associated with post-mortem muscle tenderness. The invention further relates to oligonucleotides useful in identifying the genotype of bovines as it relates to the CAST locus polymorphic site. The invention also encompasses computer-assisted methods and systems for improving the production efficiency for livestock having marketably tender meat using multiple data, and in particular the genotype of the animals as it relates to the CAST SNP. These methods of the invention encompass obtaining a genetic sample from each animal in a herd of livestock, determining the genotype of each animal with respect to specific quality traits as defined by a panel of at least two single polynucleotide polymorphisms (SNPs), one SNP corresponding to a site between exons 5 and 6 of the bovine CAST locus, grouping animals with like genotypes, and optionally, further sub-grouping animals based on like phenotypes.

摘要翻译： 本发明涉及在编码钙蛋白酶抑制蛋白的牛CAST基因座中识别单核苷酸多态性（SNP），其中SNP的等位变异是与死后肌肉触痛相关的G / C逆转。 本发明还涉及可用于鉴定与CAST基因座多态性位点相关的牛的基因型的寡核苷酸。 本发明还包括计算机辅助方法和系统，用于使用多种数据，特别是与CAST SNP相关的动物的基因型，来提高具有市售的嫩肉的家畜的生产效率。 本发明的这些方法包括从家畜群体中的每只动物获得遗传样品，根据由至少两个单个多核苷酸多态性（SNP）的一组确定的特定质量性状确定每只动物的基因型，一个SNP对应 到牛CAST基因座的外显子5和6之间的位点，分组具有相似基因型的动物，以及任选地，基于类似表型进一步分组动物。