公开(公告)号：US20110081449A1

公开(公告)日：2011-04-07

申请号：US12995398

申请日：2009-06-12

申请人： Lena De Leenheer ,  Kim Martha Jozefa Frooninckx ,  Francois Alice Alphonse Heroufosse

发明人： Lena De Leenheer ,  Kim Martha Jozefa Frooninckx ,  Francois Alice Alphonse Heroufosse

IPC分类号： C13K11/00 ,  A23L1/09 ,  A23L1/302 ,  A23K1/16 ,  A23L1/304 ,  A23L1/305

CPC分类号： C07H3/06 ,  A23K20/163 ,  A23L5/00 ,  A23L29/244 ,  A23L29/30 ,  A23L33/10 ,  A23L33/125 ,  A23L33/21 ,  C08B37/0054 ,  C12P19/18

摘要： The invention relates to a process for the production of a fructooligosaccharide (FOS) composition on industrial scale, comprising the steps of: a) providing a raw material containing sucrose; b) bringing the raw material together with an enzyme to form a mixture, whereby the enzyme has an endo-inulinase activity and/or a fructosyltransferase activity and is in free, non-immobilized form; c) exposing the mixture to conditions whereby FOS-forming can take place for an amount of time such that the FOS in the mixture constitutes at least 45 wt. % of the total amount of carbohydrates in the mixture, thereby forming the FOS composition; d) optionally deactivating the enzyme; e) recovering the FOS composition. The invention further relates to Fructooligosaccharide composition (FC) wherein: a portion of at most 2 wt. % of the carbohydrates in the FC consists of oligosaccharides that have a degree of polymerisation (DP) of 7 or more; and at least 56 wt. % of the fructooligosaccharides in the FC have a DP of 3 as measured in HPLC; and the FC is obtained from sucrose in an enzymatic process, whereby no enzyme is used that is obtained from a genetically-modified organism.

摘要翻译： 本发明涉及一种以工业规模生产低聚果糖（FOS）组合物的方法，包括以下步骤：a）提供含有蔗糖的原料; b）将原料与酶一起形成混合物，由此该酶具有内切菊糖酶活性和/或果糖基转移酶活性，并且是游离的，非固定的形式; c）将混合物暴露于其中FOS形成可以发生一段时间的条件，使得混合物中的FOS构成至少45重量％ 混合物中碳水化合物总量的百分比，从而形成FOS组合物; d）任选地使酶失活; e）回收FOS组合物。 本发明还涉及低聚果糖组合物（FC），其中：至少2wt。 FC中的碳水化合物的％由具有7或更高的聚合度（DP）的寡糖组成; 和至少56wt。 FC中的低聚果糖的％的DP为3，在HPLC中测得; 并且在酶处理中从蔗糖获得FC，由此不能使用从遗传修饰的生物获得的酶。