公开(公告)号：US06673222B1

公开(公告)日：2004-01-06

申请号：US09809108

申请日：2001-03-16

申请人： Sadaksharasundaram S. Papavinasam ,  William D. Gould ,  Robert W. Revie ,  Fraser A. MacLeod ,  Michael Attard

发明人： Sadaksharasundaram S. Papavinasam ,  William D. Gould ,  Robert W. Revie ,  Fraser A. MacLeod ,  Michael Attard

IPC分类号： G01N27327

CPC分类号： C12Q1/005 ,  C12Q1/04 ,  G01N2333/90212

摘要： A biological activity probe for the detection on-line of the presence of sulphide including an enzyme electrode; a counter electrode; a reference electrode; and if desired a corrosion electrode. The enzyme electrode includes an immobilised source of sulfide oxidase enzyme, together with a cofactor such as 1,1′-dimethylferrocene, immobilised in a water permeable non-conducting binder on a biologically inert electrically conducting substrate. The immobilised source of sulfide oxidase enzyme is either a chemoautotropic microorganism such as a Thiobacillus thioparus, or enzyme material recovered from a culture of a Thiobacillus thioparus. The probe when connected to a suitable electrical system is capable of identifying microbiological activity resulting in microbiologically influenced corrosion; microbiological activity not resulting in microbiologically influenced corrosion; microbiological inactivity, coupled with non-microbiologically influenced corrosion; and microbiological inactivity; and no corrosion.

摘要翻译： 用于在线检测包括酶电极的硫化物存在的生物活性探针; 对电极 参考电极; 并且如果需要，腐蚀电极。 酶电极包括固定在生物惰性导电基底上的透水非导电粘合剂中的硫化物氧化酶的固定源和辅助因子如1,1'-二甲基二茂铁。 硫化物氧化酶的固定化源是化学自旋性微生物，例如硫代硫杆菌（Thiobacillus thioparus）或从硫杆菌硫代硫杆菌培养物中回收的酶物质。 连接到合适的电气系统时的探头能够识别导致微生物影响的腐蚀的微生物活性; 微生物活性不会导致微生物影响的腐蚀; 微生物不活动，加上非微生物学影响的腐蚀; 和微生物不活动; 无腐蚀。

公开(公告)号：US4800959A

公开(公告)日：1989-01-31

申请号：US122814

申请日：1987-11-19

申请人： J. William F. Costerton ,  Francene Cusack ,  Fraser A. MacLeod

发明人： J. William F. Costerton ,  Francene Cusack ,  Fraser A. MacLeod

IPC分类号： C09K8/514 ,  C09K8/90 ,  C12P19/04 ,  E21B43/22 ,  E21B33/138

CPC分类号： C09K8/90 ,  C09K8/514 ,  C12P19/04 ,  C12R1/22 ,  C12R1/40 ,  Y10S435/852 ,  Y10S435/877

摘要： A microbial process is provided for selectively plugging a high permeability stratum or zone in a subterranean reservoir. Starved bacteria of reduced size are injected into the zone. A poor-nutrient media is either simultaneously or subsequently thereafter injected into the zone to substantially uniformly resuscitate the starved bacteria. Thereupon, the bacteria regain full cell size, proliferate, and commence production of biofilm-forming exocellular polysaccharides. The biofilm is functional to selectively seal off the high permeability zone of the formation and reduce aqueous flow through the zone.

摘要翻译： 提供微生物过程以选择性地堵塞地下储层中的高渗透性地层或区域。 减小尺寸的饥饿细菌注入该区域。 不良营养培养基同时或之后随后注入该区域以基本均匀地复苏饥饿的细菌。 因此，细菌恢复全细胞大小，增殖，并开始生产生物膜形成外细胞多糖。 生物膜可用于选择性地密封地层的高渗透性区域并减少通过该区域的水流。