公开(公告)号：US07045597B2

公开(公告)日：2006-05-16

申请号：US10250644

申请日：2001-01-04

申请人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi ,  Masao Shibata ,  Motoki Kuhara

发明人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi ,  Masao Shibata ,  Motoki Kuhara

IPC分类号： C07K14/435 ,  A61K39/00

CPC分类号： C07K14/705 ,  C07K16/28

摘要： A mast cell surface antigen, DNA thereof and an antibody against the antigen are provided. The amino acid sequence of this mast cell surface antigen is the translation of the coding region of its DNA. The base sequence of this DNA has been clarified in the following manner. Namely, mast cells obtained by incubating cord blood monocular cells are co-incubated with primary culture of fibroblasts to give connective tissue type mast cells (MC-TC). Then mRNA is extracted from this MC-TC cell extraction and a cDNA library is constructed therefrom. Immunological screening is carried out with the use of anti-MC-TC antiserum and the base sequence of the positive clone thus obtained is identified. Owing to the clarification of the amino acid sequence of this mast cell antigen, it becomes possible to reveal the role of mast cells in the pathology of allergic diseases and thus an antibody against mast cells can be easily obtained.

摘要翻译： 提供肥大细胞表面抗原，其DNA和针对抗原的抗体。 该肥大细胞表面抗原的氨基酸序列是其DNA编码区的翻译。 该DNA的碱基序列已经以下述方式进行了说明。 即，将通过孵育脐带血单核细胞获得的肥大细胞与成纤维细胞的原代培养物共温育以产生结缔组织型肥大细胞（MC-TC）。 然后从该MC-TC细胞提取中提取mRNA，并由其构建cDNA文库。 使用抗MC-TC抗血清进行免疫筛选，鉴定得到的阳性克隆的碱基序列。 由于这种肥大细胞抗原的氨基酸序列的澄清，可以揭示肥大细胞在过敏性疾病的病理学中的作用，因此可以容易地获得抗肥大细胞的抗体。

公开(公告)号：US06255107B1

公开(公告)日：2001-07-03

申请号：US09229932

申请日：1999-01-13

申请人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi

发明人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi

IPC分类号： C12N512

CPC分类号： C07K14/705 ,  C07K16/28

摘要： The invention is related to antibodies which specifically react with connective tissue type-human mast cells, a production method of the antibodies, hybridomas which produce the antibodies, a production method of the hybridomas and antigen proteins recognized by the antibodies. After cord blood cells were cultured in the presence of SCF and IL-6, they were further cocultured with primary culture of human skin fibroblasts, and connective tissue type-human mast cells were thus obtained. A rat was immunized using the cells, hybridomas were prepared and selected by an ordinary method, and novel monoclonal antibodies were harvested from the culture supernatant of the selected hybridomas. The monoclonal antibodies specifically reacted with connective tissue type-human mast cells.

摘要翻译： 本发明涉及与结缔组织型 - 人肥大细胞特异性反应的抗体，抗体的制备方法，产生抗体的杂交瘤，由抗体识别的杂交瘤和抗原蛋白的制备方法。 在SCF和IL-6存在下培养脐带血细胞后，与人皮肤成纤维细胞的原代培养物进一步共培养，得到结缔组织型人类肥大细胞。 使用细胞免疫大鼠，通过常规方法制备杂交瘤并选择，并从所选择的杂交瘤的培养上清液中收获新的单克隆抗体。 单克隆抗体与结缔组织型 - 人类肥大细胞特异性反应。

公开(公告)号：US20060276631A1

公开(公告)日：2006-12-07

申请号：US11415575

申请日：2006-05-02

申请人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi ,  Masao Shibata ,  Motoki Kuhara

发明人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi ,  Masao Shibata ,  Motoki Kuhara

IPC分类号： C07K14/74 ,  C07K16/28 ,  C07H21/04

CPC分类号： C07K14/705 ,  C07K16/28

摘要： A mast cell surface antigen, DNA thereof and an antibody against the antigen are provided. The amino acid sequence of this mast cell surface antigen is the translation of the coding region of its DNA. The base sequence of this DNA has been clarified in the following manner. Namely, mast cells obtained by incubating cord blood monocular cells are co-incubated with primary culture of fibroblasts to give connective tissue type mast cells (MC-TC). Then mRNA is extracted from this MC-TC cell extraction and a cDNA library is constructed therefrom. Immunological screening is carried out with the use of anti-MC-TC antiserum and the base sequence of the positive clone thus obtained is identified. Owing to the clarification of the amino acid sequence of this mast cell antigen, it becomes possible to reveal the role of mast cells in the pathology of allergic diseases and thus an antibody against mast cells can be easily obtained.

摘要翻译： 提供肥大细胞表面抗原，其DNA和针对抗原的抗体。 该肥大细胞表面抗原的氨基酸序列是其DNA编码区的翻译。 该DNA的碱基序列已经以下述方式进行了说明。 即，将通过孵育脐带血单核细胞获得的肥大细胞与成纤维细胞的原代培养物共温育以产生结缔组织型肥大细胞（MC-TC）。 然后从该MC-TC细胞提取中提取mRNA，并由其构建cDNA文库。 使用抗MC-TC抗血清进行免疫筛选，鉴定得到的阳性克隆的碱基序列。 由于这种肥大细胞抗原的氨基酸序列的澄清，可以揭示肥大细胞在过敏性疾病的病理学中的作用，因此可以容易地获得抗肥大细胞的抗体。

公开(公告)号：US06258564B1

公开(公告)日：2001-07-10

申请号：US09363787

申请日：1999-07-29

申请人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi

发明人： Makoto Kawai ,  Tadashi Okada ,  Fukiko Atsumi

IPC分类号： C12N1502

CPC分类号： C07K14/705 ,  C07K16/28

摘要： The invention is related to antibodies which specifically react with connective tissue type-human mast cells, a production method of the antibodies, hybridomas which produce the antibodies, a production method of the hybridomas and antigen proteins recognized by the antibodies. After cord blood cells were cultured in the presence of SCF and IL-6, they were further cocultured with primary culture of human skin fibroblasts, and connective tissue type-human mast cells were thus obtained. A rat was immunized using the cells, hybridomas were prepared and selected by an ordinary method, and novel monoclonal antibodies were harvested from the culture supernatant of the selected hybridomas. The monoclonal antibodies specifically reacted with connective tissue type-human mast cells.

摘要翻译： 本发明涉及与结缔组织型 - 人肥大细胞特异性反应的抗体，抗体的制备方法，产生抗体的杂交瘤，由抗体识别的杂交瘤和抗原蛋白的制备方法。 在SCF和IL-6存在下培养脐带血细胞后，与人皮肤成纤维细胞的原代培养物进一步共培养，得到结缔组织型人类肥大细胞。 使用细胞免疫大鼠，通过常规方法制备杂交瘤并选择，并从所选择的杂交瘤的培养上清液中收获新的单克隆抗体。 单克隆抗体与结缔组织型 - 人类肥大细胞特异性反应。