METHOD OF PRODUCING HETERODIMER DERIVATIVE OF PROTEIN PHOSPHATASE TYPE 2A ENZYME
    1.
    发明申请
    METHOD OF PRODUCING HETERODIMER DERIVATIVE OF PROTEIN PHOSPHATASE TYPE 2A ENZYME 失效
    蛋白质磷酸酯酶2A型酶的制备方法

    公开(公告)号:US20090093018A1

    公开(公告)日:2009-04-09

    申请号:US12094067

    申请日:2005-11-16

    CPC classification number: C12N9/16

    Abstract: The purpose of the invention is to provide an activated protein phosphatase 2A (PP2A) in large quantities with high purity by a genetic engineering and to provide a method for producing a heterodimer derivative of PP2A which comprises infecting insect cultured cells with a baculovirus in which a cDNA encoding the catalytic subunit of PP2A carrying a first tag is integrated together with another baculovirus in which a cDNA encoding the A subunit of PP2A carrying a second tag is integrated, incubating the infected cells, disrupting the incubated cells to obtain a disrupted cell suspension, and then purifying the disrupted cell suspension with a solid phase carrying a substance capable of binding to the first tag and another solid phase carrying a substance capable of binding to the second tag, characterized in that the insect cells infected with the baculovirus are incubated at a temperature of from 18 to 22° C.

    Abstract translation: 本发明的目的是通过基因工程提供大量高纯度的活化蛋白磷酸酶2A(PP2A),并提供生产PP2A异二聚体衍生物的方法,其包括用杆状病毒感染昆虫培养的细胞,其中 编码携带第一标签的PP2A的催化亚基的cDNA与另一种杆状病毒整合在一起,其中编码携带第二标签的PP2A的A亚基的cDNA整合,孵育感染的细胞,破坏孵育的细胞以获得破坏的细胞悬浮液, 然后用携带能够结合第一标签的物质的固相和另一种携带能够结合第二标签的物质的固相纯化破碎的细胞悬浮液,其特征在于用杆状病毒感染的昆虫细胞在 温度为18〜22℃

    Method of producing heterodimer derivative of protein phosphatase type 2A enzyme
    2.
    发明授权
    Method of producing heterodimer derivative of protein phosphatase type 2A enzyme 失效
    蛋白磷酸酶2A型异源二聚体衍生物的制备方法

    公开(公告)号:US07745191B2

    公开(公告)日:2010-06-29

    申请号:US12094067

    申请日:2005-11-16

    CPC classification number: C12N9/16

    Abstract: The purpose of the invention is to provide an activated protein phosphatase 2A (PP2A) in large quantities with high purity by a genetic engineering and to provide a method for producing a heterodimer derivative of PP2A which comprises infecting insect cultured cells with a baculovirus in which a cDNA encoding the catalytic subunit of PP2A carrying a first tag is integrated together with another baculovirus in which a cDNA encoding the A subunit of PP2A carrying a second tag is integrated, incubating the infected cells, disrupting the incubated cells to obtain a disrupted cell suspension, and then purifying the disrupted cell suspension with a solid phase carrying a substance capable of binding to the first tag and another solid phase carrying a substance capable of binding to the second tag, characterized in that the insect cells infected with the baculovirus are incubated at a temperature of from 18 to 22° C.

    Abstract translation: 本发明的目的是通过基因工程提供大量高纯度的活化蛋白磷酸酶2A(PP2A),并提供生产PP2A异二聚体衍生物的方法,其包括用杆状病毒感染昆虫培养的细胞,其中 编码携带第一标签的PP2A的催化亚基的cDNA与另一种杆状病毒整合在一起,其中编码携带第二标签的PP2A的A亚基的cDNA整合,孵育感染的细胞,破坏孵育的细胞以获得破坏的细胞悬浮液, 然后用携带能够结合第一标签的物质的固相和另一种携带能够结合第二标签的物质的固相纯化破碎的细胞悬浮液,其特征在于用杆状病毒感染的昆虫细胞在 温度为18〜22℃

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