公开(公告)号：US08729338B2

公开(公告)日：2014-05-20

申请号：US13496468

申请日：2010-09-17

申请人： Gino Boussiengui-Boussiengui ,  Jens Kossmann

发明人： Gino Boussiengui-Boussiengui ,  Jens Kossmann

IPC分类号： A01H5/00 ,  C12N15/113

CPC分类号： C12N15/8245 ,  C12N9/88

摘要： A method of modifying at least one carbohydrate in a tissue of a plant is described. The method is typically applied to a sugarcane plant of the genus Saccharum method and includes the steps of inserting into a plant cell a gene silencing cassette which includes nucleic acid operably linked to transcription elements such as a monocotyledonous promoter for transcribing the nucleic acid in a plant cell, wherein transcription of the nucleic acid decreases activity of UMP synthase. The method further includes the steps of regenerating a transgenic plant from the plant cell and producing the tissue with increased carbohydrate content. Vectors for use therefor, as well as a transformed plant cell and a transgenic plant or plant part containing or derived from a transformed plant cell are also described.

摘要翻译： 描述了修饰植物组织中的至少一种碳水化合物的方法。 该方法通常应用于蔗糖方法的甘蔗植物，并且包括将基因沉默盒插入到植物细胞中的步骤，该基因沉默盒包括可操作地连接到转录元件的核酸，例如用于转录植物中的核酸的单子叶启动子 细胞，其中所述核酸的转录降低UMP合酶的活性。 该方法还包括从植物细胞再生转基因植物并产生具有增加的碳水化合物含量的组织的步骤。 还描述了用于其的载体，以及转化的植物细胞和含有或衍生自转化的植物细胞的转基因植物或植物部分。

公开(公告)号：US20120180161A1

公开(公告)日：2012-07-12

申请号：US13496468

申请日：2010-09-17

申请人： Gino Boussiengui-Boussiengui ,  Jens Kossmann

发明人： Gino Boussiengui-Boussiengui ,  Jens Kossmann

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N5/10

CPC分类号： C12N15/8245 ,  C12N9/88

摘要： A method of modifying at least one carbohydrate in a tissue of a plant is described. The method is typically applied to a sugarcane plant of the genus Saccharum method and includes the steps of inserting into a plant cell a gene silencing cassette which includes nucleic acid operably linked to transcription elements such as a monocotyledonous promoter for transcribing the nucleic acid in a plant cell, wherein transcription of the nucleic acid decreases activity of UMP synthase. The method further includes the steps of regenerating a transgenic plant from the plant cell and producing the tissue with increased carbohydrate content. Vectors for use therefor, as well as a transformed plant cell and a transgenic plant or plant part containing or derived from a transformed plant cell are also described.

摘要翻译： 描述了修饰植物组织中的至少一种碳水化合物的方法。 该方法通常应用于蔗糖方法的甘蔗植物，并且包括将基因沉默盒插入到植物细胞中的步骤，该基因沉默盒包括可操作地连接到转录元件的核酸，例如用于转录植物中的核酸的单子叶启动子 细胞，其中所述核酸的转录降低UMP合酶的活性。 该方法还包括从植物细胞再生转基因植物并产生具有增加的碳水化合物含量的组织的步骤。 还描述了用于其的载体，以及转化的植物细胞和含有或衍生自转化的植物细胞的转基因植物或植物部分。