公开(公告)号：US07001749B2

公开(公告)日：2006-02-21

申请号：US10654890

申请日：2003-09-05

申请人： Toshiteru Komatsu ,  Yoshitaka Senda ,  Goro Ujita

发明人： Toshiteru Komatsu ,  Yoshitaka Senda ,  Goro Ujita

IPC分类号： C12P7/64

CPC分类号： C12N11/00

摘要： Provided is a method for regenerating an immobilized enzyme for lipolysis which has been used for lipolysis, thereby exhibiting a reduced activity, which includes washing the immobilized enzyme with a solvent; controlling an equilibrium concentration of fatty acids in the solvent; removing the washed immobilized enzyme therefrom, and contacting the resulting immobilized enzyme with a fresh enzyme, wherein the fresh enzyme adsorbs onto the immobilized enzyme. According to the preferred methods of the present invention, it is possible to make effective use of the residual activity of an immobilized enzyme spent for lipolysis and regenerate the spent immobilized enzyme to have a performance similar to that before the lipolysis at a low cost using less amount of liquid waste.

摘要翻译： 本发明提供一种再利用脂肪分解用固定化酶的方法，其用于脂肪分解，从而表现出降低的活性，其包括用溶剂洗涤固定化酶; 控制溶剂中脂肪酸的平衡浓度; 从其中除去洗涤的固定化酶，并使得到的固定化酶与新鲜酶接触，其中新鲜酶吸附到固定化酶上。 根据本发明的优选方法，可以有效利用用于脂肪分解的固定化酶的残留活性，并使用较少的成本再生废消化的固定化酶以具有与脂肪分解之前相似的性能 液体废物量。