公开(公告)号：US20060154262A1

公开(公告)日：2006-07-13

申请号：US11032766

申请日：2005-01-11

申请人： Paul Fisher ,  Habib Boukerche

发明人： Paul Fisher ,  Habib Boukerche

IPC分类号： C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  C12P21/06 ,  A61K48/00

CPC分类号： C07K14/4748 ,  C12Q1/6886 ,  C12Q2600/118

摘要： The present invention relates to the discovery, identification and characterization of an eight genes that are differentially expressed as a consequence of metastatic progression in human melanoma cells. Six of the identified metastasis elevated genes (MEG genes), encode for known proteins (MEG-1 through MEG-6). However, two of the identified genes, referred to herein as MEG-7 and MEG-8, represent novel genes. The present invention relates to methods for inhibiting the metastatic potential of cancer cells through inhibition of the MEG genes/gene products, as well as diagnostic methods for determining the metastatic potential of cells. The invention further relates to novel MEG-7 and MEG-8 nucleotides, host cell expression systems, MEG-7 and MEG-8 proteins, fusion proteins, and antibodies to the MEG-7 and MEG-8. The present invention also relates to the discovery that inhibition of the mda-9 gene (also referred to as syntenin), a gene found to be upregulated in metastatic cancer cells, results in a decrease in the invasive and migratory properties of such cells. Thus, the present invention relates also to methods and compositions for inhibiting mda-9/syntenin activity.

摘要翻译： 本发明涉及在人黑素瘤细胞中差异表达为转移进展的结果的8个基因的发现，鉴定和表征。 确定的转移基因（MEG基因）中的六个，编码已知蛋白质（MEG-1至MEG-6）。 然而，在本文中称为MEG-7和MEG-8的两个鉴定的基因代表新的基因。 本发明涉及通过抑制MEG基因/基因产物来抑制癌细胞的转移潜力的方法以及用于确定细胞转移潜能的诊断方法。 本发明还涉及新的MEG-7和MEG-8核苷酸，宿主细胞表达系统，MEG-7和MEG-8蛋白，融合蛋白和针对MEG-7和MEG-8的抗体。 本发明还涉及发现在转移性癌细胞中被发现上调的基因的mda-9基因（也称为syntenin）的抑制导致这些细胞的侵入性和迁移性质的降低。 因此，本发明还涉及抑制mda-9 / syntenin活性的方法和组合物。