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    Method for Enhancing N-acetylglucosamine Production through glcK Knockout of Bacillus subtilis
    1.
    发明申请
    Method for Enhancing N-acetylglucosamine Production through glcK Knockout of Bacillus subtilis 有权
    通过glcK敲除枯草芽孢杆菌来增强N-乙酰氨基葡萄糖生产的方法

    公开(公告)号：US20170009267A1

    公开(公告)日：2017-01-12

    申请号：US14934166

    申请日：2015-11-06

    申请人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

    发明人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

    IPC分类号： C12P19/26 ,  C12N15/75

    CPC分类号： C12P19/26 ,  C12N15/75 ,  C12R1/125

    摘要： The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h−1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.

    摘要翻译： 本发明提供了通过使用具有glcK敲除的重组枯草芽孢杆菌来增强N-乙酰葡糖胺产生的方法。 本发明通过敲除编码葡糖激酶的glcK基因来增强GlcNAc的产生，从而消除GlcNAc磷酸化成GlcNAc-6-P。 具有glcK敲除的重组枯草芽孢杆菌上清液中GlcNAc的比生长速率和含量分别为0.15h-1和3.0g / L，分别为没有glcK敲除的对照菌株的2.32倍和2.14倍。 本发明的重组枯草芽孢杆菌可能用于GlcNAc的工业生产。