公开(公告)号：US06613564B2

公开(公告)日：2003-09-02

申请号：US09740903

申请日：2000-12-21

申请人： Hirokazu Ohbayashi ,  Yuriko Kitano

发明人： Hirokazu Ohbayashi ,  Yuriko Kitano

IPC分类号： G01N33535

CPC分类号： G01N33/535 ,  Y10S435/96 ,  Y10S435/964

摘要： The invention relates to a complex of enzyme, protein and carrier comprising two or more molecules of an enzyme conjugated through amino group or other group to a carrier such as polylysine, and a protein (for example, antibody) with a specific binding potency to other substance(s) (for example, antigen) conjugated to at least one of said two or more molecules of the enzyme, which enables accurate assay of a trace amount of a substance at a high sensitivity.

摘要翻译： 本发明涉及一种酶，蛋白质和载体的复合物，其包含两个或更多个通过氨基或其它基团共轭的分子与载体如聚赖氨酸的分子，以及具有与其它结合特异性结合力的蛋白质（例如抗体） 与酶的所述两种或更多种分子中的至少一种缀合的物质（例如抗原），其能够以高灵敏度精确测定痕量的物质。

公开(公告)号：US07235369B2

公开(公告)日：2007-06-26

申请号：US10608025

申请日：2003-06-30

申请人： Hirokazu Ohbayashi ,  Yuriko Kitano

发明人： Hirokazu Ohbayashi ,  Yuriko Kitano

IPC分类号： G01N33/53

CPC分类号： G01N33/535 ,  Y10S435/96 ,  Y10S435/964

摘要： A complex of enzyme, protein and carrier comprising two or more molecules of an enzyme conjugated through an amino group or other group to a carrier such as polylysine, and a protein (for example, antibody) with a specific binding potency to other substance(s) (for example, antigen) conjugated to at least one of said two or more molecules of the enzyme. This complex enables accurate assay of a trace amounts of a substance at a high sensitivity.

摘要翻译： 包含通过氨基或其它基团共轭的两个或更多分子的酶，载体如聚赖氨酸的蛋白质和载体的复合物，以及与其它物质具有特异性结合力的蛋白质（例如抗体） ）（例如，抗原），其与酶的所述两个或更多个分子中的至少一个缀合。 该复合物能够以高灵敏度精确测定痕量的物质。

公开(公告)号：US06252053B1

公开(公告)日：2001-06-26

申请号：US09268748

申请日：1999-03-17

申请人： Hirokazu Ohbayashi ,  Yuriko Kitano ,  Takashi Kitoh

发明人： Hirokazu Ohbayashi ,  Yuriko Kitano ,  Takashi Kitoh

IPC分类号： C12N1106

CPC分类号： G01N33/535 ,  Y10S435/96

摘要： The present invention relates to an enzyme-antibody complex where one or more molecule(s) of enzyme into which a maleimide group or a thiol group is introduced is/are covalently conjugated to a carrier (polylysine, aminodextyan and so forth) in which a thiol group when a maleimide group is introduced into the enzyme or to a maleimide group when a thiol group is introduced into the enzyme via them, and a maleimide group is introduced into at least one amino group remaining in the above complex and is covalently conjugated to a maleimide group of the above complex via a thiol group obtained by reduction of antibody fragment or antibody. The resulting complex is useful as an enzyme-labeled antibody particularly in immunohistochemistry and in enzyme immunoassay and makes an immunoassay with high sensitivity possible.

摘要翻译： 本发明涉及一种酶 - 抗体复合物，其中引入了马来酰亚胺基团或硫醇基团的一个或多个分子的酶共价结合到载体（聚赖氨酸，氨基代谢等）上，其中 当硫醇基通过它们引入酶中时，当将马来酰亚胺基团引入酶或马来酰亚胺基团时，硫醇基团，并且将马来酰亚胺基团导入至上述复合物中残留的至少一个氨基，并共价共轭 通过还原抗体片段或抗体而获得的硫醇基团的上述络合物的马来酰亚胺基团。 所得的复合物可用作酶标记的抗体，特别是在免疫组织化学和酶免疫测定中，并且可以进行具有高灵敏度的免疫测定。