公开(公告)号：US06861258B2

公开(公告)日：2005-03-01

申请号：US10085388

申请日：2002-02-28

申请人： Kevin J. Kayser ,  Ho-Shin Park ,  John J. Kilbane, II

发明人： Kevin J. Kayser ,  Ho-Shin Park ,  John J. Kilbane, II

IPC分类号： C12N1/21 ,  C12N15/74 ,  C12Q1/68 ,  C12P21/00

CPC分类号： C12R1/20 ,  C12N15/74 ,  C12Q1/6897

摘要： A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.

摘要翻译： 一种用于在嗜热宿主中引入和稳定异源和重组基因的方法，其中定义可检测的宿主特征的特征基因从嗜热宿主中失活或缺失，导致表达不存在可检测宿主特征的经修饰的嗜热宿主。 将感兴趣的DNA片段与完整特征基因一起插入修饰的嗜热性宿主中，由此将可检测的宿主特征恢复到嗜热宿主，从而使得能够使用质粒载体检测和确认成功的转化并将DNA片段整合到 嗜热宿主的染色体。