Oligonucleotide, method and system for detecting antibiotic resistance-mediating genes in microorganisms by means of real-time PCR
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    发明申请
    Oligonucleotide, method and system for detecting antibiotic resistance-mediating genes in microorganisms by means of real-time PCR 审中-公开
    通过实时PCR检测微生物中抗生素抗性介导基因的寡核苷酸,方法和系统

    公开(公告)号:US20050059064A1

    公开(公告)日:2005-03-17

    申请号:US10928647

    申请日:2004-08-26

    IPC分类号: C12Q1/68 C07H21/04 C12P19/34

    CPC分类号: C12Q1/689

    摘要: An oligonucleotide, a method and a system for detecting antibiotic resistance-mediating genes in microorganisms by means of real-time PCR, comprising: the use of a first primer nucleotide sequence (A) which is selected from the group of sequences consisting of SEQ# 1-4, the use of a second primer nucleotide sequence (B) which is selected from the group of sequences consisting of SEQ# 5-8, with the sequences 1 and 5, 2 and 6, 3 and 7, and 4 and 8 being used as primer pairs, and the use of at least one first dye (C) for detecting the PCR-amplified DNA, and their use, in particular on a biochip.

    摘要翻译: 一种用于通过实时PCR检测微生物中抗生素抗性介导基因的寡核苷酸,方法和系统,包括:使用选自SEQ ID NO:1的序列组成的序列组的第一引物核苷酸序列(A) 如图1-4所示,使用选自SEQ ID NO:5-8的序列的第二引物核苷酸序列(B)与序列1和5,2和6,3和7,以及4和8 用作引物对,以及使用至少一种第一染料(C)来检测PCR扩增的DNA,以及它们在生物芯片上的用途。