公开(公告)号：US07674918B2

公开(公告)日：2010-03-09

申请号：US10599767

申请日：2005-04-06

申请人： Gerald Arthur Smith ,  Darren William Watson ,  Howard Paul Voorheis

发明人： Gerald Arthur Smith ,  Darren William Watson ,  Howard Paul Voorheis

IPC分类号： C07D311/88

CPC分类号： C09B11/28 ,  C09B11/22 ,  C09B69/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

摘要： A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, alkaryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O− or S−; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through. the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.

摘要翻译： 包含具有式（I）的呫吨衍生的荧光团的荧光染料，其中R1，R2，R3，R4，R5和R6独立地选自H，烷基，烷氧基，醇，醚，烯基，烯氧基，芳基，烷芳基，芳烷基 和酰氨基，除了分别键合到Y，Y 1或Y 2时，R 1，R 4和/或R 5不为H; X是O-或S-; Y，Y 1，Y 2，Y 3，Y 4和Y 5中的至少一个是用于共价键合染料的基团，任选地通过。 使用偶联剂到靶分子，否则为H.染料可以共价连接到靶分子以形成复合物，并且染料和/或复合物可用于细胞分析技术，特别是pH测量和 迁移动力学分析。

公开(公告)号：US20100256399A1

公开(公告)日：2010-10-07

申请号：US12694510

申请日：2010-01-27

申请人： Gerald Arthur Smith ,  Darren William Watson ,  Howard Paul Voorheis

发明人： Gerald Arthur Smith ,  Darren William Watson ,  Howard Paul Voorheis

IPC分类号： C07D311/88

CPC分类号： C09B11/28 ,  C09B11/22 ,  C09B69/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

摘要： A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O− or S−; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through, the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.

摘要翻译： 一种荧光染料，其包含具有式（I）的呫吨衍生的荧光团，其中R1，R2，R3，R4，R5和R6独立地选自H，烷基，烷氧基，醇，醚，烯基，烯氧基，芳基，芳烷基和酰氨基 ，除了分别键合到Y，Y 1或Y 2时，R 1，R 4和/或R 5不为H; X是O-或S-; Y，Y 1，Y 2，Y 3，Y 4和Y 5中的至少一个是用于将染料共价键合的基团，任选地通过使用偶联剂与靶分子共用，否则为H.染料可以共价 连接到靶分子以形成复合物，染料和/或复合物可用于细胞分析技术，特别是pH测量和迁移动力学分析。

公开(公告)号：US08101655B2

公开(公告)日：2012-01-24

申请号：US12694510

申请日：2010-01-27

申请人： Gerald Arthur Smith ,  Darren William Watson ,  Howard Paul Voorheis

发明人： Gerald Arthur Smith ,  Darren William Watson ,  Howard Paul Voorheis

IPC分类号： A61K31/35 ,  C07D311/88

CPC分类号： C09B11/28 ,  C09B11/22 ,  C09B69/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

摘要： A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O− or S−; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through, the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.

摘要翻译： 一种荧光染料，其包含具有式（I）的呫吨衍生的荧光团，其中R1，R2，R3，R4，R5和R6独立地选自H，烷基，烷氧基，醇，醚，烯基，烯氧基，芳基，芳烷基和酰氨基 ，除了分别键合到Y，Y 1或Y 2时，R 1，R 4和/或R 5不为H; X是O-或S-; Y，Y 1，Y 2，Y 3，Y 4和Y 5中的至少一个是用于将染料共价键合的基团，任选地通过使用偶联剂与靶分子共用，否则为H.染料可以共价 连接到靶分子以形成复合物，染料和/或复合物可用于细胞分析技术，特别是pH测量和迁移动力学分析。