公开(公告)号：US06954225B2

公开(公告)日：2005-10-11

申请号：US10063322

申请日：2002-04-11

Applicant: Hsiao-Ping Chen

Inventor： Hsiao-Ping Chen

IPC: G06T7/20 ,  H04N5/14 ,  H04N7/18

CPC classification number: H04N5/144 ,  G06T7/254

Abstract: A method detects activity within a fixed view by comparing first and second images. The first and second images respectively include a plurality of blocks, and each of the blocks having a plurality of pixels. The method including: calculating an average value A for each block of the second image, each of the average values A is equal to an average of differences between pixels of one of the blocks of the second image and pixels of a corresponding block of the first image; calculating a mean difference B between average pixel values of the first and second images; calculating a difference V between average value A and mean difference B for each block of the second image, and selecting blocks of the second image that have corresponding differences V greater than a threshold value; and determining whether there is any object moving within the fixed view according to the selected blocks.

Abstract translation: 方法通过比较第一和第二图像来检测固定视图内的活动。 第一和第二图像分别包括多个块，并且每个块具有多个像素。 该方法包括：计算第二图像的每个块的平均值A，每个平均值A等于第二图像的一个块的像素与第一图像的相应块的像素之间的差的平均值 图片; 计算第一和第二图像的平均像素值之间的平均差B; 计算第二图像的每个块的平均值A和平均差B之间的差V，以及选择具有大于阈值的对应差异V的第二图像的块; 以及根据所选择的块确定在固定视图内是否存在任何物体移动。

公开(公告)号：US20100264040A1

公开(公告)日：2010-10-21

申请号：US12826717

申请日：2010-06-30

Applicant: SHAU-CHUN WANG ,  Hsueh-Chia Chang ,  Hsiao-Ping Chen ,  Chun-Ming Chang

Inventor： SHAU-CHUN WANG ,  Hsueh-Chia Chang ,  Hsiao-Ping Chen ,  Chun-Ming Chang

IPC: B01D43/00

CPC classification number: B03C5/02

Abstract: The present invention provides a method for concentrating particles or molecules and an apparatus thereof. The apparatus comprises a substrate, a conducting granule having nano-pores or nano-channels capable of permitting ion permeation, an electrolyte solution comprising counter-ions having an opposite electric property to the conducting granule, and an external field. Wherein, particles or molecules to be concentrated have an identical electric property as the conducting granule at a predefined pH value, and are added into the electrolyte solution with the predefined pH value. While the external electric field is applied across the reservoir where the conducting granule is sitting, the counter-ions exit from the nano-pores or nano-channels and such that a transient ion super-concentration phenomenon occurs at an ejecting pole on the conducting granule so as to concentrate the particles or molecules. Hence the present invention has potential application in bead-based molecular assays.

Abstract translation: 本发明提供了一种浓缩颗粒或分子的方法及其装置。 该装置包括：具有纳米孔的导电颗粒或能够允许离子渗透的纳米通道，包含与导电颗粒具有相反电性的抗衡离子的电解质溶液和外部场。 其中，要浓缩的颗粒或分子具有与导电颗粒相同的电特性，并且以预定的pH值加入到电解质溶液中。 当外部电场施加在导电颗粒所在的储存器上时，反离子从纳米孔或纳米通道离开，并且在导电颗粒上的喷射极处发生瞬态离子超浓度现象 以便集中颗粒或分子。 因此，本发明在基于珠的分子测定中具有潜在的应用。

公开(公告)号：US07265777B2

公开(公告)日：2007-09-04

申请号：US10248811

申请日：2003-02-21

Applicant: Hsiao-Ping Chen ,  Chou-Ming Chang

Inventor： Hsiao-Ping Chen ,  Chou-Ming Chang

IPC: H04N7/18

CPC classification number: G06T9/004 ,  G06T9/20

Abstract: A motion detection method is used to abstract multiple moving objects. The motion detection method uses a first frame and a second frame to locate the moving objects appearing in a fixed scene. The method includes calculating a difference of each pixel between the first frame and the second frame, comparing each difference with a first threshold value for generating a plurality of first contours, comparing each difference with a second threshold value for generating a plurality of second contours, picking any of the second contours which overlapped with the first contours for generating corresponding moving blocks, determining whether the pixels located outside the moving blocks are background pixels, and comparing the parameter of each background pixel located inside the moving blocks with its corresponding background value for determining pixels related to the moving objects.

Abstract translation: 运动检测方法用于抽取​​多个移动物体。 运动检测方法使用第一帧和第二帧来定位出现在固定场景中的移动物体。 该方法包括计算第一帧和第二帧之间的每个像素的差值，将每个差值与用于产生多个第一轮廓的第一阈值进行比较，将每个差值与用于产生多个第二轮廓的第二阈值进行比较， 选择与第一轮廓重叠的任何第二轮廓以产生相应的移动块，确定位于移动块外的像素是否是背景像素，以及将位于移动块内的每个背景像素的参数与其对应的背景值进行比较 确定与移动物体相关的像素。

公开(公告)号：US20090277792A1

公开(公告)日：2009-11-12

申请号：US12383893

申请日：2009-03-30

Applicant: Shau-Chun Wang ,  Hsueh-Chia Chang ,  Hsiao-Ping Chen ,  Hsien-Hung Wei ,  Chun-Ching Yu ,  Min-Hsuan Tsai

Inventor： Shau-Chun Wang ,  Hsueh-Chia Chang ,  Hsiao-Ping Chen ,  Hsien-Hung Wei ,  Chun-Ching Yu ,  Min-Hsuan Tsai

IPC: G01N27/447 ,  G01N27/453

CPC classification number: B03C5/02

Abstract: The present invention discloses a method for concentrating charged particles and an apparatus thereof. The method comprises: providing a substrate comprising a reservoir; disposing a conducting granule in the reservoir, the conducting granule being negatively charged or positively charged and comprising nano-pores or nano-channels capable of permitting ion permeation; disposing a buffer solution in the reservoir, the buffer solution comprising counter-ions having an opposite electric property to the conducting granule; adding the charged particles into the buffer solution, the charged particles being co-ions having an identical electric property as the conducting granule; and applying an external electric field on the conducting granule. While the external electric field is applied on the conducting granule, the counter-ions exit from the nano-pores or nano-channels and have a nonuniform concentration on a surface of the conducting granule such that a transient ion super-concentration phenomenon occurs at an ejecting pole on the conducting granule. Hence the present invention has potential application in bead-based molecular assays.

Abstract translation: 本发明公开了一种用于浓缩带电粒子的方法及其装置。 该方法包括：提供包括储存器的基底; 在储存器中设置导电颗粒，导电颗粒带负电荷或带正电荷，并包含能够允许离子渗透的纳米孔或纳米通道; 在储存器中设置缓冲溶液，所述缓冲溶液包含与导电颗粒具有相反电性质的抗衡离子; 将带电粒子加入到缓冲溶液中，带电粒子是与导电颗粒具有相同电性质的共离子; 并在导电颗粒上施加外部电场。 当外部电场施加在导电颗粒上时，抗衡离子从纳米孔或纳米通道离开，并且在导电颗粒的表面上具有不均匀的浓度，使得瞬时离子超浓度现象发生在 在导电颗粒上喷射极。 因此，本发明在基于珠的分子测定中具有潜在的应用。

公开(公告)号：US20080316854A1

公开(公告)日：2008-12-25

申请号：US12076378

申请日：2008-03-18

Applicant: Shau-Chun Wang ,  Hsiao-Ping Chen ,  Chia Yu Lee ,  Hsueh-Chia Chang

Inventor： Shau-Chun Wang ,  Hsiao-Ping Chen ,  Chia Yu Lee ,  Hsueh-Chia Chang

IPC: B01F11/02

CPC classification number: B01F13/0076 ,  B01L3/502715

Abstract: A microfluid mixer is provided. The non-linear electrokineticsis is applied to the design of the microfluid mixer. The microfluid mixer comprises a first and a second microfluidic elements, a mixing reservoir, and a micro channel unit, wherein the micro channel unit has at least two control channels for respectively connecting the first and the second microfluidic elements and the mixing reservoir. When two microfluids are mixed in the mixing reservoir, the electro-osmosis fluid field of the microfluids in the control channel of the mixing reservoir is changed by applying AC signal, such that powerful chaotic mixing effect is therefore produced by the two microfluids in the mixing reservoir.

Abstract translation: 提供微流体混合器。 非线性电动力学应用于微流体混合器的设计。 微流体混合器包括第一和第二微流体元件，混合储存器和微通道单元，其中微通道单元具有用于分别连接第一和第二微流体元件和混合储存器的至少两个控制通道。 当在混合容器中混合两个微流体时，通过施加AC信号来改变混合容器的控制通道中的微流体的电渗流体场，从而在混合中由两个微流体产生强大的混沌混合效应 水库