公开(公告)号：US20090286220A1

公开(公告)日：2009-11-19

申请号：US12437291

申请日：2009-05-07

申请人： Sergey V. Sheleg ,  Hugh L. Hixon, JR. ,  Bruce Cohen ,  Sergei A. Svarovsky

发明人： Sergey V. Sheleg ,  Hugh L. Hixon, JR. ,  Bruce Cohen ,  Sergei A. Svarovsky

IPC分类号： A01N1/02

CPC分类号： A01N1/0221

摘要： A method for hypothermic preservation of biological tissue for later recovery to a viable state includes flushing the biological tissue with a gas mixture of sulfur hexafluoride or xenon and oxygen. The sulfur hexafluoride or xenon is in a concentration in the mixture between about 75 mole percent to 95 mole percent. The method includes pressurizing the biological tissue, preferably isothermically, with the mixture to a pressure that will form clathrates inside the biological tissue at a desired storage temperature in a range of about +1° C. to about +5° C. The method includes a step of cooling the biological tissue, preferably isobarically, to the desired storage temperature. Optional steps for further cooling to no colder than about −20° C. and for depressurization are provided as well as steps for recovering the hypothermically preserved biological tissue to a viable state, preferably using a recovery gas mixture.

摘要翻译： 生物组织的低温保存方法以便稍后恢复到可行状态包括用六氟化硫或氙气和氧气的混合物冲洗生物组织。 六氟化硫或氙的混合浓度为约75摩尔％至95摩尔％。 该方法包括将生物组织，优选等温地加压至在约+ 1℃至约+ 5℃的范围内的所需储存温度下将在生物组织内形成包合物的压力。该方法包括 将生物组织，优选地等温地冷却至所需的储存温度的步骤。 提供进一步冷却至不低于约-20℃并用于减压的可选步骤，以及用于将低温保存的生物组织回收至可行状态的步骤，优选使用回收气体混合物。