FLUORESCENT MICROSCOPE FOR OBSERVING MULTIPLE FLUORESCENT IMAGES, FLUORESCENT IMAGE SURVEYING METHOD USING THE SAME, AND MULTIPLE FLUORESCENT IMAGE OBSERVING SYSTEM
    2.
    发明申请
    FLUORESCENT MICROSCOPE FOR OBSERVING MULTIPLE FLUORESCENT IMAGES, FLUORESCENT IMAGE SURVEYING METHOD USING THE SAME, AND MULTIPLE FLUORESCENT IMAGE OBSERVING SYSTEM 有权
    用于观察多个荧光图像的荧光显微镜,使用其的荧光图像测量方法和多个荧光图像观察系统

    公开(公告)号:US20130201322A1

    公开(公告)日:2013-08-08

    申请号:US13809319

    申请日:2011-08-18

    IPC分类号: G02B21/00

    摘要: A fluorescent microscope for observing multiple fluorescent images includes: a first optical module comprising a first light source for supplying first excitation light having a first wavelength, a first excitation filter for selectively transmitting the first excitation light supplied from the first light source, a first dichroic filter for reflecting the first excitation light having passed through the first excitation filter toward the survey object, an objective lens for condensing the first excitation light reflected by the first dichroic filter and transferring the condensed first excitation light to the survey object, a second dichroic filter for reflecting first radiation light radiated from the survey object, a first radiation filter for selectively transmitting the first radiation light reflected by the second dichroic filter, and a first image acquisition unit for acquiring an image by using the first radiation light having passed through the first radiation filter to be supplied; and a second optical module comprising a second light source for supplying second excitation light having a second wavelength, a second excitation filter for selectively transmitting the second excitation light supplied from the second light source, a second radiation filter passing through the second excitation filter and irradiated to the survey object to be radiated, to selectively transmitting the second radiation light EM2 having passed through the objective lens, the first dichroic filter, and the second excitation filter, and a second image acquisition unit for acquiring an image by using the second radiation light having passed through the second radiation filter to be supplied.

    摘要翻译: 用于观察多个荧光图像的荧光显微镜包括:第一光学模块,包括用于提供具有第一波长的第一激发光的第一光源,用于选择性地透射从第一光源提供的第一激发光的第一激发滤光器,第一二色性 滤光器,用于将通过第一激发滤光器的第一激发光朝向测量对象反射;物镜,用于会聚由第一分色滤光器反射的第一激发光并将聚集的第一激发光转印到测量对象;第二二向色滤光片 用于反射从所述测量对象辐射的第一辐射光,用于选择性地透射由所述第二分色滤光器反射的所述第一辐射光的第一辐射滤光器;以及第一图像获取单元,用于通过使用已经穿过所述第一 辐射过滤器为sup 合并 以及第二光学模块,包括用于提供具有第二波长的第二激发光的第二光源,用于选择性地透射从第二光源提供的第二激发光的第二激发滤光器,穿过第二激发滤光器并照射的第二辐射滤光片 到要被辐射的测量对象,以选择性地透过已经通过物镜的第二辐射光EM2,第一二向色滤光器和第二激发滤光器,以及第二图像获取单元,用于通过使用第二辐射光获取图像 已通过要供应的第二辐射滤波器。

    Fluorescent microscope for observing multiple fluorescent images, fluorescent image surveying method using the same, and multiple fluorescent image observing system
    4.
    发明授权
    Fluorescent microscope for observing multiple fluorescent images, fluorescent image surveying method using the same, and multiple fluorescent image observing system 有权
    用于观察多个荧光图像的荧光显微镜,使用其的荧光图像测量方法和多个荧光图像观察系统

    公开(公告)号:US09395527B2

    公开(公告)日:2016-07-19

    申请号:US13809319

    申请日:2011-08-18

    IPC分类号: G02B21/00 G02B21/16 G01N21/64

    摘要: A fluorescent microscope for observing multiple fluorescent images includes: a first optical module comprising a first light source for supplying first excitation light having a first wavelength, a first excitation filter for selectively transmitting the first excitation light supplied from the first light source, a first dichroic filter for reflecting the first excitation light having passed through the first excitation filter toward the survey object, an objective lens for condensing the first excitation light reflected by the first dichroic filter and transferring the condensed first excitation light to the survey object, a second dichroic filter for reflecting first radiation light radiated from the survey object, a first radiation filter for selectively transmitting the first radiation light reflected by the second dichroic filter, and a first image acquisition unit for acquiring an image by using the first radiation light having passed through the first radiation filter to be supplied; and a second optical module comprising a second light source for supplying second excitation light having a second wavelength, a second excitation filter for selectively transmitting the second excitation light supplied from the second light source, a second radiation filter passing through the second excitation filter and irradiated to the survey object to be radiated, to selectively transmitting the second radiation light EM2 having passed through the objective lens, the first dichroic filter, and the second excitation filter, and a second image acquisition unit for acquiring an image by using the second radiation light having passed through the second radiation filter to be supplied.

    摘要翻译: 用于观察多个荧光图像的荧光显微镜包括:第一光学模块,包括用于提供具有第一波长的第一激发光的第一光源,用于选择性地透射从第一光源提供的第一激发光的第一激发滤光器,第一二色性 滤光器,用于将通过第一激发滤光器的第一激发光朝向测量对象反射;物镜,用于会聚由第一分色滤光器反射的第一激发光并将聚集的第一激发光转印到测量对象;第二二向色滤光片 用于反射从所述测量对象辐射的第一辐射光,用于选择性地透射由所述第二分色滤光器反射的所述第一辐射光的第一辐射滤光器;以及第一图像获取单元,用于通过使用已经穿过所述第一 辐射过滤器为sup 合并 以及第二光学模块,包括用于提供具有第二波长的第二激发光的第二光源,用于选择性地透射从第二光源提供的第二激发光的第二激发滤光器,穿过第二激发滤光器并照射的第二辐射滤光片 到要被辐射的测量对象,以选择性地透过已经通过物镜的第二辐射光EM2,第一二向色滤光器和第二激发滤光器,以及第二图像获取单元,用于通过使用第二辐射光获取图像 已通过要供应的第二辐射滤波器。