公开(公告)号：US20100203572A1

公开(公告)日：2010-08-12

申请号：US12664246

申请日：2008-06-13

申请人： Werner Lehmann ,  Alexander Böhm ,  Kai Grossmann ,  Rico Hiemann ,  Jörg Nitschke ,  Stefan Rödiger

发明人： Werner Lehmann ,  Alexander Böhm ,  Kai Grossmann ,  Rico Hiemann ,  Jörg Nitschke ,  Stefan Rödiger

IPC分类号： C12Q1/02 ,  G01N21/76 ,  C12Q1/00

CPC分类号： G01N33/542 ,  C12Q1/6818 ,  G01N33/543

摘要： The invention relates to a method comprising the following steps: a) use of a support which has at least two different microparticle populations immobilized thereon; b) measuring the fluorescence of the support from step a) with an optical resolution 1, said resolution 1 permitting differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers and determination of the localized position of individual immobilized microparticles; c) contacting the support from step a) with the sample to be analyzed; d) performing at least one additional measurement of the fluorescence of the support during or after contacting in accordance with step c) with a resolution 2; e) assigning the fluorescence values measured with resolution 2 to the individual microparticle singlets, doublets, triplets, multiplets and monolayers locally identified on the support in accordance with step b) and assigned to a particular acceptor molecule population; f) determining the change in fluorescence. The method is used to determine reaction kinetics, concentrations and affinities of analytes in samples.

摘要翻译： 本发明涉及包括以下步骤的方法：a）使用其上固定有至少两个不同微粒群的载体; b）用光学分辨率1测量来自步骤a）的载体的荧光，所述分辨率1允许微粒单峰，双峰，三重峰，多重点和单层的分化和测定个体固定化微粒的局部位置; c）使来自步骤a）的载体与待分析的样品接触; d）根据步骤c）或其接触期间或之后对分析物2的荧光进行至少一次附加测量; e）根据步骤b）将分辨率2测量的荧光值分配给在载体上局部鉴定的单个微粒单体，双峰，三重峰，多重峰和单层，并分配给特定的受体分子群体; f）确定荧光的变化。 该方法用于确定样品中分析物的反应动力学，浓度和亲和力。