公开(公告)号：US5238835A

公开(公告)日：1993-08-24

申请号：US600429

申请日：1990-10-19

申请人： Bryan D. McKersie ,  Tissa Senaratna ,  Steve Bowley ,  J. Derek Bewley ,  Daniel C. W. Brown

发明人： Bryan D. McKersie ,  Tissa Senaratna ,  Steve Bowley ,  J. Derek Bewley ,  Daniel C. W. Brown

IPC分类号： A01C1/00 ,  A01H4/00

CPC分类号： A01C1/00 ,  A01H4/00 ,  Y10S47/09

摘要： A process for inducing desiccation tolerance in an in vitro formed plant embryo to enable production of viable artificial seeds is disclosed. The process comprises culturing in vitro plant embryos, stimulating the embryos to proceed through globular and a heart shaped stage into an elongate-torpedo shaped stage and early cotyledon stage, inducing the embryos as early as the torpedo-shaped stage with a source of abscisic acid at an effective concentration of abscisic acid and for a sufficient period of time to cause expression of desiccation tolerance which includes change in cellular metabolism, electron transport processes and oxidation-reduction reactions in the embryos and drying the induced embryos to provide stable viable embryos.

摘要翻译： 公开了一种用于在体外成熟植物胚中诱导干燥耐受性以使得能够生产活的人造种子的方法。 该过程包括培养体外植物胚胎，刺激胚胎通过球状和心形阶段进入细长鱼雷形状阶段和早期子叶阶段，早在具有脱落酸源的鱼雷形状阶段诱导胚胎 在脱落酸的有效浓度和足够的时间段引起干燥耐受性的表达，其包括胚胎中的细胞代谢，电子传递过程和氧化 - 还原反应的变化，并干燥诱导的胚胎以提供稳定的活的胚胎。