公开(公告)号：US20070263954A1

公开(公告)日：2007-11-15

申请号：US11583409

申请日：2006-10-18

申请人： James Hillendahl ,  David Waldbeser

发明人： James Hillendahl ,  David Waldbeser

IPC分类号： G02B6/00

CPC分类号： G01N21/253 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

摘要： Biochemical assays that are performed in cuvettes and in the wells of multi-well plates and that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the cuvette or well. Emission light produced by the excitation is then collected by a collimating lens and converted to a signal that is compiled by conventional software for analysis. The optical fiber and collimating lens can either be on the same side of the receptacle (generally the open side) or on opposite sides, i.e., one above and the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle. Illumination systems are also disclosed in which a ultraviolet, visible, or near-infrared light source is optically coupled to an optical fiber.

摘要翻译： 通过照射和检测系统增强了在比色皿和多孔板的孔中进行的并且利用激发和发光作为检测标签的生化测定，该照明和检测系统通过透射激发光的光纤提供激发光 光源到比色皿或井。 由激发产生的发射光然后由准直透镜收集并转换为由常规软件编译的信号用于分析。 光纤和准直透镜可以位于插座的同一侧（通常为开放侧）或相对的两侧，即一个在另一个之上。 当光纤和准直透镜都在容器的开口侧时，它们被布置成使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者都在 与垂直于容器口部的轴线呈锐角。 还公开了照明系统，其中紫外线，可见光或近红外光源光学耦合到光纤。

公开(公告)号：US20100184616A1

公开(公告)日：2010-07-22

申请号：US12686832

申请日：2010-01-13

申请人： James Hillendahl ,  David Waldbeser ,  Saul Saucedo ,  Timothy Andrews

发明人： James Hillendahl ,  David Waldbeser ,  Saul Saucedo ,  Timothy Andrews

IPC分类号： C40B30/04 ,  C40B60/12

CPC分类号： G01N21/6428 ,  G01N21/253 ,  G01N21/6452 ,  G01N21/648

摘要： Two-dimensional samples or sample arrays such as electrophoresis gels and microplates, containing fluorescently labeled species, are illuminated by an illumination device that includes a slab of non-autofluorescing or low-autofluorescing material shaped to receive excitation light from one or more edges and to distribute the light to emerge from an upper surface of the slab at a uniform intensity along the length and width of the upper surface.

摘要翻译： 包含荧光标记物质的二维样品或样品阵列如电泳凝胶和微孔板被照明装置照明，照明装置包括非自发荧光或低自发荧光材料板，其被成形为接收来自一个或多个边缘的激发光， 沿着上表面的长度和宽度以均匀的强度分布光从板坯的上表面出射。

公开(公告)号：US20070065074A1

公开(公告)日：2007-03-22

申请号：US11521677

申请日：2006-09-14

申请人： James Hillendahl ,  David Waldbeser

发明人： James Hillendahl ,  David Waldbeser

IPC分类号： G02B6/32

CPC分类号： G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

摘要： Biochemical assays of samples in receptacles, in gels, in blots, in arrays and the like, and that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the sample. Emission light produced by the excitation is then collected by a lens and converted to a signal that is compiled by conventional software for analysis. The optical fiber transfixes (passes through) the lens via a slot or other opening and is preferably offset from the center of the lens. The optical fiber and collecting lens can either be on the same side of the sample or on opposite sides, i.e., one above and the other below. The optical fiber and the light collecting lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle. Illumination systems are also disclosed in which a ultraviolet, visible, or near-infrared light source is optically coupled to an optical fiber.

摘要翻译： 通过照射和检测系统增强了在容器，凝胶，印迹，阵列等中的样品和利用激发和发光作为检测标签的样品的生物化学分析，其通过透射激发光的光纤提供激发光 从激发光源到样品。 然后由透镜产生的发射光被透镜收集并转换成由常规软件编译的信号用于分析。 光纤经由狭槽或其他开口将透镜经过（透过）透镜，并且优选地偏离透镜的中心。 光纤和收集透镜可以在样品的相同侧或者在相对的两侧，即一个在另一个之上。 光纤和聚光透镜被布置成使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者都与垂直于嘴的轴成锐角 容器 还公开了照明系统，其中紫外线，可见光或近红外光源光学耦合到光纤。