公开(公告)号：US07566532B1

公开(公告)日：2009-07-28

申请号：US11169287

申请日：2005-06-28

申请人： Maurice Exner ,  Jamie L. Platt

发明人： Maurice Exner ,  Jamie L. Platt

IPC分类号： C12Q1/70 ,  C12P19/34

CPC分类号： C12Q1/703

摘要： Disclosed is a method for detecting human retroviral nucleic acids such as human immunodeficiency virus type I (HIV-1) nucleic acid, human T-cell leukemia virus type I (HTLV-I) nucleic acid, and human T-cell leukemia virus type II (HTLV-II) nucleic acid in a sample. In the method, the sample is treated with reverse transcriptase to generate cDNA, and the cDNA is subsequently analyzed to detect HIV-1, HTLV-I, and HTLV-II. The method may include performing PCR and the method may utilize specific primers. In addition, the method may utilize HTLV linkers that facilitate PCR amplification and sequencing. The cDNA may be treated with restriction enzymes before or after PCR amplification to facilitate sequencing and detection of HIV-1, HTLV-I, or HTLV-II nucleic acid.

摘要翻译： 公开了用于检测人类逆转录病毒核酸如I型（HIV-1）核酸，人类T细胞白血病病毒I型（HTLV-I）核酸和II型人类T细胞白血病病毒 （HTLV-II）核酸。 在该方法中，用逆转录酶处理样品以产生cDNA，随后分析cDNA以检测HIV-1，HTLV-1和HTLV-II。 该方法可以包括进行PCR，并且该方法可以利用特异性引物。 此外，该方法可以利用促进PCR扩增和测序的HTLV接头。 可以在PCR扩增之前或之后用限制酶处理cDNA，以促进HIV-1，HTLV-I或HTLV-II核酸的测序和检测。