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    Method for Generating Transcripts
    1.
    发明申请
    Method for Generating Transcripts 审中-公开
    生成记录的方法

    公开(公告)号：US20070260053A1

    公开(公告)日：2007-11-08

    申请号：US11660952

    申请日：2005-09-23

    申请人： Francois Mallet ,  Guy Oriol ,  Jean-Philippe Pichon

    发明人： Francois Mallet ,  Guy Oriol ,  Jean-Philippe Pichon

    IPC分类号： C07H21/04 ,  C12N9/00

    CPC分类号： C12Q1/6865 ,  C12Q2521/301

    摘要： The present invention relates to a method for generating transcripts from: at least one RNA sequence to be amplified comprising a “primer” region and a region of interest, and an amplification primer comprising a promoter region, and a region capable of hybridizing to said “primer” region of the RNA sequence to be amplified, said method being carried out at constant temperature, and comprising the following steps: a) said primer is hybridized with the RNA to be amplified, b) the primer is extended by means of a reverse transcriptase enzymatic activity in order to generate a complementary deoxyribonucleic acid (cDNA) sequence of the RNA to be amplified, c) the RNA to be amplified, hybridized to said cDNA, is cleaved by means of an enzyme that has a ribonuclease H activity, so as to obtain fragments of RNA to be amplified, hybridized to said cDNA, d) the ends of said fragments of RNA to be amplified are extended by means of a reverse transcriptase and strand displacement enzyme, so as to obtain RNA-DNA/DNA hybrids, e) RNA transcripts are obtained from the RNA-DNA/DNA hybrids formed in step d), by means of an enzyme that has an RNA polymerase activity.

    摘要翻译： 本发明涉及一种从以下产生转录物的方法：至少一个待扩增的RNA序列，其包含“引物”区和感兴趣区，扩增引物包含启动子区和能与所述“ 引物“区域，所述方法在恒温下进行，并包括以下步骤：a）所述引物与待扩增的RNA杂交，b）引物通过反向扩增 转录酶酶活性，以产生要扩增的RNA的互补脱氧核糖核酸（cDNA）序列，c）通过具有核糖核酸酶H活性的酶将待扩增的与所述cDNA杂交的RNA切割，因此 以获得要扩增的RNA片段，与所述cDNA杂交，d）通过逆转录酶和链置换酶扩增待扩增的所述RNA片段的末端，以便 获得RNA-DNA / DNA杂交体，e）通过具有RNA聚合酶活性的酶从步骤d）中形成的RNA-DNA / DNA杂交体获得RNA转录物。

    METHOD FOR IN VITRO DIAGNOSIS OR PROGNOSIS OF TESTICULAR CANCER
    2.
    发明申请
    METHOD FOR IN VITRO DIAGNOSIS OR PROGNOSIS OF TESTICULAR CANCER 审中-公开
    用于体外诊断或预后特异性癌症的方法

    公开(公告)号：US20100330581A1

    公开(公告)日：2010-12-30

    申请号：US12918166

    申请日：2009-03-10

    申请人： Maud Arsac ,  Bertrand Bonnaud ,  Francois Mallet ,  Jean-Philippe Pichon

    发明人： Maud Arsac ,  Bertrand Bonnaud ,  Francois Mallet ,  Jean-Philippe Pichon

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/702 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/158

    摘要： The invention relates to a method for in vitro diagnosis or prognosis of testicular cancer which comprises a step of detecting the presence or absence of at least one expression product from at least one nucleic acid sequence selected from the sequences identified in SEQ ID NOS: 1 to 6 or from the sequences which exhibit at least 99% identity with one of the sequences identified in SEQ ID NOS: 1 to 6, to isolated nucleic acid sequences and to the use thereof as a testicular cancer marker.

    摘要翻译： 本发明涉及一种用于体外诊断或睾丸癌预后的方法，其包括从至少一种选自SEQ ID NO：1中鉴定的序列的核酸序列检测至少一种表达产物的存在或不存在的步骤， 6或来自与SEQ ID NO：1至6中鉴定的一个序列具有至少99％同一性的序列，或与分离的核酸序列及其作为睾丸癌标志物的用途的序列。