公开(公告)号：US5721096A

公开(公告)日：1998-02-24

申请号：US255471

申请日：1994-06-08

申请人： Sotirios K. Karathanasis ,  John A.A. Ladias ,  Jeffrey N. Rottman ,  Russell L. Widom

发明人： Sotirios K. Karathanasis ,  John A.A. Ladias ,  Jeffrey N. Rottman ,  Russell L. Widom

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6897

摘要： Activation of the apolipoprotein AI (apoAI) gene in mammalian liver cells has been studied, as described herein, and shown to depend on synergistic interactions between nuclear proteins which bind to three distinct sites within a hepatocyte-specific transcriptional enhancer located between nucleotides -222 to -110 5' of the human apoAI gene (relative to the transcription start site). Two proteins, the apoAI regulatory protein-1 and the retinoic acid receptor RXR.alpha., have been shown to regulate expression of the apoAI gene.

摘要翻译： 如本文所述已经研究了哺乳动物肝细胞中载脂蛋白AI（apoAI）基因的活化，并且显示出依赖于核苷酸之间的协同相互作用的核蛋白之间的协同相互作用，所述核蛋白结合位于核苷酸-222至 -110 5'的人apoAI基因（相对​​于转录起始位点）。 已经显示两种蛋白质，即载脂蛋白A调节蛋白-1和视黄酸受体RXRα调节apoAI基因的表达。