公开(公告)号：US20070154901A1

公开(公告)日：2007-07-05

申请号：US11452434

申请日：2006-06-14

申请人： Hans Thogersen ,  Michael Etzerodt ,  Thor Holtet ,  Niels Graversen ,  Jette Kastrup ,  Bettina Nielsen ,  Ingrid Larsen

发明人： Hans Thogersen ,  Michael Etzerodt ,  Thor Holtet ,  Niels Graversen ,  Jette Kastrup ,  Bettina Nielsen ,  Ingrid Larsen

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K14/47

CPC分类号： C12N15/62 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4726 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/41 ,  C07K2319/50 ,  C07K2319/73 ,  C07K2319/95

摘要： The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.

摘要翻译： 本发明涉及使用衍生自tetranectin蛋白家族的多肽结构元件的三聚体多肽的设计及其在合理的从头设计和生产多功能分子的用途，包括在蛋白质文库技术中应用多功能分子， 如噬菌体展示技术，诊断和治疗系统，如人类基因治疗和成像。 三聚体多肽被构建为包含至少一种与至少一个异源部分共价连接的四联蛋白三聚结构元件（TTSE）的单体多肽构建体，所述TTSE能够与两个其它TTSE形成稳定的复合物; 或作为如上所述由两个单体多肽构建体组成的寡聚体，并且其包含三个TTSE或三个TTSE的多重，或由三个单体多肽构建体组成。