公开(公告)号：US08765196B2

公开(公告)日：2014-07-01

申请号：US14006474

申请日：2012-02-24

申请人： Jun He ,  Jichuan Kang ,  Jianian Yang ,  Yixin Qian

发明人： Jun He ,  Jichuan Kang ,  Jianian Yang ,  Yixin Qian

IPC分类号： A61K36/16

CPC分类号： C07D493/22

摘要： Disclosed is a method for separating and purifying Ginkgolide C from root bark of ginkgo. The method comprises: (1) extracting the root bark of ginkgo with ethanol; (2) concentrating the resulting extract under vacuum to remove ethanol; (3) separating the concentrate by macroporous resin column chromatography; (4) after the concentrate being loaded on the column, washing the column with pure water to remove impurities, and then eluting the column with an ethanol solution; (5) concentrating the eluate under vacuum to dryness to obtain a yellow crude extract; (6) heating the crude extract in water to boiling to form a solution, and then refrigerating the solution; (7) concentrating the supernatant solution and filtering under vacuum to obtain a mixed crude crystal of ginkgolides; (8) dissolving the crude crystal in ethanol to form a supersaturated solution, refrigerating and crystallizing the solution to remove Ginkgolides A and B; (9) concentrating and recrystallizing the mother liquor to obtain a crystal of Ginkgolide C; and (10) recrystallizing the crystal with ethanol several times to obtain a high-purity crystal of Ginkgolide C.

摘要翻译： 公开了银杏根皮中银杏内酯C的分离纯化方法。 该方法包括：（1）用乙醇提取银杏根皮; （2）将所得提取物在真空下浓缩以除去乙醇; （3）通过大孔树脂柱层析分离浓缩物; （4）将浓缩物装入柱后，用纯水洗涤柱以除去杂质，然后用​​乙醇溶液洗脱柱; （5）将真空浓缩洗脱液至干，得到黄色粗提物; （6）将水中的粗提物加热至沸腾形成溶液，然后冷却溶液; （7）浓缩上清液，真空过滤，得到银杏内酯混合粗晶; （8）将粗晶体溶解在乙醇中以形成过饱和溶液，冷却并结晶溶液以除去银杏内酯A和B; （9）浓缩和重结晶母液得到银杏内酯C的晶体; 和（10）用乙醇重结晶晶体数次，得到银杏内酯C的高纯度晶体。

公开(公告)号：US20140039202A1

公开(公告)日：2014-02-06

申请号：US14006474

申请日：2012-02-24

申请人： Jun He ,  Jichuan Kang ,  Jianian Yang ,  Yixin Qian

发明人： Jun He ,  Jichuan Kang ,  Jianian Yang ,  Yixin Qian

IPC分类号： C07D493/22

CPC分类号： C07D493/22

摘要： Disclosed is a method for separating and purifying Ginkgolide C from root bark of ginkgo. The method comprises: (1) extracting the root bark of ginkgo with ethanol; (2) concentrating the resulting extract under vacuum to remove ethanol; (3) separating the concentrate by macroporous resin column chromatography; (4) after the concentrate being loaded on the column, washing the column with pure water to remove impurities, and then eluting the column with an ethanol solution; (5) concentrating the eluate under vacuum to dryness to obtain a yellow crude extract; (6) heating the crude extract in water to boiling to form a solution, and then refrigerating the solution; (7) concentrating the supernatant solution and filtering under vacuum to obtain a mixed crude crytal of ginkgolides; (8) dissolving the crude crystal in ethanol to form a supersaturated solution, refrigerating and crystallizing the solution to remove Ginkgolides A and B; (9) concentrating and recrystallizing the mother liquor to obtain a crystal of Ginkgolide C; and (10) recrystallizing the crystal with ethanol several times to obtain a high-purity crystal of Ginkgolide C.

摘要翻译： 公开了银杏根皮中银杏内酯C的分离纯化方法。 该方法包括：（1）用乙醇提取银杏根皮; （2）将所得提取物在真空下浓缩以除去乙醇; （3）通过大孔树脂柱层析分离浓缩物; （4）将浓缩物装入柱后，用纯水洗涤柱以除去杂质，然后用​​乙醇溶液洗脱柱; （5）将真空浓缩洗脱液至干，得到黄色粗提物; （6）将水中的粗提物加热至沸腾形成溶液，然后冷却溶液; （7）浓缩上清溶液，真空过滤，得到银杏内酯混合粗血浆; （8）将粗晶体溶解在乙醇中以形成过饱和溶液，冷却并结晶溶液以除去银杏内酯A和B; （9）浓缩和重结晶母液得到银杏内酯C的晶体; 和（10）用乙醇重结晶晶体数次，得到银杏内酯C的高纯度晶体。