公开(公告)号：US5972670A

公开(公告)日：1999-10-26

申请号：US005397

申请日：1998-01-09

申请人： Xu Feng ,  Randy M. Berka ,  Jill Angela Wahleithner

发明人： Xu Feng ,  Randy M. Berka ,  Jill Angela Wahleithner

IPC分类号： C12N9/02

CPC分类号： C12N9/0061

摘要： The present invention relates to mutants of a blue multi-copper oxidase, comprising (a) a substitution of one or more amino acid residues with other amino acid residues, (b) an insertion of one or more amino acid residues and/or (c) a deletion of one or more amino acid residues, wherein the substitution, insertion or deletion is carried out at a position which is located no greater than 15 .ANG. from a Type I (T1) copper site. The present invention also relates to nucleic acid constructs comprising a nucleic acid sequence encoding the mutants of the present invention, host cells comprising the construct of the present invention, and methods for producing mutants of the present invention.

摘要翻译： 本发明涉及蓝色多铜氧化酶的突变体，其包含（a）一个或多个氨基酸残基与其它氨基酸残基的取代，（b）插入一个或多个氨基酸残基和/或（c ）一个或多个氨基酸残基的缺失，其中取代，插入或缺失在位于I型（T1）铜位点不超过15 ANGSTROM的位置进行。 本发明还涉及包含编码本发明突变体的核酸序列，包含本发明构建体的宿主细胞和本发明的突变体的制备方法的核酸构建体。

公开(公告)号：US5770419A

公开(公告)日：1998-06-23

申请号：US706037

申请日：1996-08-30

申请人： Xu Feng ,  Randy M. Berka ,  Jill Angela Wahleithner

发明人： Xu Feng ,  Randy M. Berka ,  Jill Angela Wahleithner

IPC分类号： C12N9/02

CPC分类号： C12N9/0061

摘要： The present invention relates to mutants of a blue multi-copper oxidase, comprising (a) a substitution of one or more amino acid residues with other amino acid residues, (b) an insertion of one or more amino acid residues and/or (c) a deletion of one or more amino acid residues, wherein the substitution, insertion or deletion is carried out at a position which is located no greater than 15 .ANG. from a Type I (T1) copper site. The present invention also relates to nucleic acid constructs comprising a nucleic acid sequence encoding the mutants of the present invention, host cells comprising the construct of the present invention, and methods for producing mutants of the present invention.

摘要翻译： 本发明涉及蓝色多铜氧化酶的突变体，其包含（a）一个或多个氨基酸残基与其它氨基酸残基的取代，（b）插入一个或多个氨基酸残基和/或（c ）一个或多个氨基酸残基的缺失，其中取代，插入或缺失在位于I型（T1）铜位点不超过15 ANGSTROM的位置进行。 本发明还涉及包含编码本发明突变体的核酸序列，包含本发明构建体的宿主细胞和本发明的突变体的制备方法的核酸构建体。