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公开(公告)号：US20140328900A1
公开(公告)日：2014-11-06
申请号：US14234919
申请日：2012-08-03
IPC分类号： C07K14/64
摘要： A process for preparing human relaxin-2 having the following amino acid sequence:A chain: (SEQ ID NO: 1) pGlu-Leu-Tyr-Ser-Ala-Leu-Ala-Asn-Lys-Cys-Cys-His- Val-Gly-Cys-Thr-Lys-Arg-Ser-Leu-Ala-Arg-Phe-Cys B chain: (SEQ ID NO: 2) Asp-Ser-Trp-Met-Glu-Glu-Val-Ile-Lys-Leu-Cys-Gly- Arg-Glu-Leu-Val-Arg-Ala-Gln-Ile-Ala-Ile-Cys-Gly- Met-Ser-Thr-Trp-Ser; comprising the following steps:providing the amino acids necessary for the synthesis of the A and B chains with usual protective groups, wherein the cysteines are employed as trityl-protected amino acids (L-Cys(Trt)-OH);effecting a chromatographic purification of the individual chains A and B after the solid state synthesis;followed by the simultaneous folding and combination of the individual chains A and B in ammonium hydrogencarbonate buffer at pH 7.9 to 8.4; andsubsequent purification of the relaxin-2 formed.
摘要翻译： 具有以下氨基酸序列的人松弛素-2的制备方法：A链：（SEQ ID NO：1）pGlu-Leu-Tyr-Ser-Ala-Leu-Ala-Asn-Lys-Cys-Cys-His-Val -Gly-Cys-Thr-Lys-Arg-Ser-Leu-Ala-Arg-Phe-Cys B链：（SEQ ID NO：2）Asp-Ser-Trp-Met-Glu-Glu-Val-Ile-Lys- Leu-Cys-Gly-Arg-Glu-Leu-Val-Arg-Ala-Gln-Ile-Ala-Ile-Cys-Gly-Met-Ser-Thr-Trp-Ser; 包括以下步骤：用通常的保护基提供合成A和B链所必需的氨基酸，其中半胱氨酸用作三苯甲基保护的氨基酸（L-Cys（Trt）-OH））; 在固态合成后进行单链A和B的色谱纯化; 然后在pH 7.9至8.4的同时折叠和组合各个链A和B在碳酸氢铵缓冲液中; 并随后纯化形成的松弛素-2。
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公开(公告)号：US09434780B2
公开(公告)日：2016-09-06
申请号：US14234919
申请日：2012-08-03
IPC分类号： C07K14/64
摘要： A process for preparing human relaxin-2 having the following amino acid sequence: A chain: (SEQ ID NO: 1) pGlu-Leu-Tyr-Ser-Ala-Leu-Ala-Asn-Lys-Cys-Cys-His- Val-Gly-Cys-Thr-Lys-Arg-Ser-Leu-Ala-Arg-Phe-Cys B chain: (SEQ ID NO: 2) Asp-Ser-Trp-Met-Glu-Glu-Val-Ile-Lys-Leu-Cys-Gly- Arg-Glu-Leu-Val-Arg-Ala-Gln-Ile-Ala-Ile-Cys-Gly- Met-Ser-Thr-Trp-Ser; comprising the following steps: providing the amino acids necessary for the synthesis of the A and B chains with usual protective groups, wherein the cysteines are employed as trityl-protected amino acids (L-Cys(Trt)-OH); effecting a chromatographic purification of the individual chains A and B after the solid state synthesis; followed by the simultaneous folding and combination of the individual chains A and B in ammonium hydrogencarbonate buffer at pH 7.9 to 8.4; and subsequent purification of the relaxin-2 formed.
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