公开(公告)号：US06537790B1

公开(公告)日：2003-03-25

申请号：US09585468

申请日：2000-06-02

申请人： Kae Kishimoto ,  Yuko Kobayashi ,  Yoshiki Takashima ,  Ayumu Inoue

发明人： Kae Kishimoto ,  Yuko Kobayashi ,  Yoshiki Takashima ,  Ayumu Inoue

IPC分类号： C12N916

CPC分类号： C12P17/10 ,  C12N9/18

摘要： The present invention provides a novel gene encoding a protein having an excellent catalyst ability for producing (S)-N-substituted cyclic imino acid represented by formula (2): and provides a novel method for producing (S)-N-substituted cyclic imino acid represented by formula (2) by an asymmetric hydrolization of the N-substituted cyclic imino acid ester represented by formula (1): in a manner of gene engineering technology utilizing the novel gene provided.

摘要翻译： 本发明提供了编码由式（2）表示的（S）-N-取代的环状亚氨酸具有优异的催化能力的蛋白质的新基因：提供了一种制备（S）-N-取代的环状亚氨基 由式（2）表示的酸通过式（1）表示的N-取代的环状亚氨基酸酯的不对称水解：以使用所提供的新基因的基因工程技术的方式。

公开(公告)号：US06828129B2

公开(公告)日：2004-12-07

申请号：US10114116

申请日：2002-05-07

申请人： Kae Kishimoto ,  Yuko Kobayashi ,  Yoshiki Takashima ,  Ayumu Inoue

发明人： Kae Kishimoto ,  Yuko Kobayashi ,  Yoshiki Takashima ,  Ayumu Inoue

IPC分类号： C12P1300

CPC分类号： C12P17/10 ,  C12N9/18

摘要： The present invention provides a novel gene encoding a protein having an excellent catalyst ability for producing (S)-N-substituted cyclic imino acid represented by the general formula (2) (=the (S)-cyclic imino acid (2)): and to provide a novel method for producing the (S)-cyclic imino acid (2) by an asymmetric hydrolization of the N-substituted cyclic imino acid ester represented by the general formula (1): in a manner of gene engineering technology utilizing the novel gene provided.

公开(公告)号：US06812008B2

公开(公告)日：2004-11-02

申请号：US10114115

申请日：2002-04-03

申请人： Kae Kishimoto ,  Yuko Kobayashi ,  Yoshiki Takashima ,  Ayumu Inoue

发明人： Kae Kishimoto ,  Yuko Kobayashi ,  Yoshiki Takashima ,  Ayumu Inoue

IPC分类号： C12P1500

CPC分类号： C12P17/10 ,  C12N9/18

摘要： The present invention provides a novel gene encoding a protein having an excellent catalyst ability for producing (S)-N-substituted cyclic imino acid represented by the general formula (2) (=the (S)-cyclic imino acid (2)): and to provide a novel method for producing the (S)-cyclic imino acid (2) by an asymmetric hydrolization of the N-substituted cyclic imino acid ester represented by the general formula (1): in a manner of gene engineering technology utilizing the novel gene provided.

摘要翻译： 本发明提供了编码具有优异的催化能力的蛋白质的新型基因，该蛋白质具有优良的通式（2）表示的（S）-N-取代的环状亚氨基酸（=（S） - 环亚氨基酸（2））的催化能力： 并且提供通过由通式（1）表示的N-取代的环亚氨基酸酯的不对称水解制备（S） - 环亚氨基酸（2）的新方法：以基因工程技术利用 提供新基因。