公开(公告)号：US20120301487A1

公开(公告)日：2012-11-29

申请号：US13575756

申请日：2011-01-24

申请人： Indraneel Mittra ,  Rekha Mannemcherril Ramesan ,  Chandra Prakash Sharma ,  Gopichettipalayam Subbaratnam Vaneshwar ,  Kavita Anirban Pal

发明人： Indraneel Mittra ,  Rekha Mannemcherril Ramesan ,  Chandra Prakash Sharma ,  Gopichettipalayam Subbaratnam Vaneshwar ,  Kavita Anirban Pal

IPC分类号： A61K39/395 ,  C07K16/18 ,  A61P29/00 ,  B82Y5/00

CPC分类号： C07K16/18 ,  A61K47/61 ,  A61K47/6939 ,  A61K2039/505 ,  C07K16/08 ,  Y10T428/2982

摘要： A process for substantially reducing levels of circulating chromatin fragments (CCFs) from a medium using binding agents such as antibodies or antibodies complexed with haemocompatible natural polymer substrates like as alginates, chitosan and pullulan to form complexed antibody-substrate nano-particulates (CNP) to bind and/or inactivate CCFs is disclosed. The amount of antibody bound to the polymer varies from 30% to 100% of activated sites in the polymer. Elevated levels of CCFs can be substantially reduced following administration of tissue damaging agents that generate apoptotic chromatin fragments by the concomitant administration of CNPs or concomitant administration of H4 antibody alone. A method of treatment is disclosed wherein therapeutic dose of CNPs, or H4 antibody alone, are administered systematically, or orally, in a delivery system to curb pathological conditions that are associated with increased burden of circulating chromatin fragments.

摘要翻译： 使用结合剂（例如抗体或抗体与诸如藻酸盐，壳聚糖和支链淀粉等血液相容性天然聚合物底物复合的抗体）从培养基中显着降低循环染色质片段（CCF）的水平的方法，以形成复合的抗体 - 底物纳米颗粒（CNP） 公开了结合和/或灭活CCF。 与聚合物结合的抗体的量在聚合物中的活化位点的30％至100％不等。 在施用组合损伤剂后，CCF的升高水平可以显着降低，所述组织损伤剂通过伴随施用CNP或单独施用H4抗体产生凋亡染色质片段。 公开了一种治疗方法，其中治疗剂量的CNP或单独的H4抗体在递送系统中系统地或口服地施用以遏制与循环染色质片段的负担增加相关的病理状况。

公开(公告)号：US09096655B2

公开(公告)日：2015-08-04

申请号：US13575756

申请日：2011-01-24

申请人： Indraneel Mittra ,  Rekha Mannemcherril Ramesan ,  Chandra Prakash Sharma ,  Gopichettipalayam Subbaratnam Bhuvaneshwar ,  Kavita Anirban Pal

发明人： Indraneel Mittra ,  Rekha Mannemcherril Ramesan ,  Chandra Prakash Sharma ,  Gopichettipalayam Subbaratnam Bhuvaneshwar ,  Kavita Anirban Pal

IPC分类号： A61K47/28 ,  A61K39/44 ,  C07K16/08 ,  A61K47/48 ,  A61K39/00

CPC分类号： C07K16/18 ,  A61K47/61 ,  A61K47/6939 ,  A61K2039/505 ,  C07K16/08 ,  Y10T428/2982

摘要： A process for substantially reducing levels of circulating chromatin fragments (CCFs) from a medium using binding agents such as antibodies or antibodies complexed with haemocompatible natural polymer substrates like as alginates, chitosan and pullulan to form complexed antibody-substrate nano-particulates (CNP) to bind and/or inactivate CCFs is disclosed. The amount of antibody bound to the polymer varies from 30% to 100% of activated sites in the polymer. Elevated levels of CCFs can be substantially reduced following administration of tissue damaging agents that generate apoptotic chromatin fragments by the concomitant administration of CNPs or concomitant administration of H4 antibody alone. A method of treatment is disclosed wherein therapeutic dose of CNPs, or H4 antibody alone, are administered systematically, or orally, in a delivery system to curb pathological conditions that are associated with increased burden of circulating chromatin fragments.

摘要翻译： 使用结合剂（例如抗体或抗体与诸如藻酸盐，壳聚糖和支链淀粉等血液相容性天然聚合物底物复合的抗体）从培养基中显着降低循环染色质片段（CCF）的水平的方法，以形成复合的抗体 - 底物纳米颗粒（CNP） 公开了结合和/或灭活CCF。 与聚合物结合的抗体的量在聚合物中的活化位点的30％至100％不等。 在施用组合损伤剂后，CCF的升高水平可以显着降低，所述组织损伤剂通过伴随施用CNP或单独施用H4抗体产生凋亡染色质片段。 公开了一种治疗方法，其中治疗剂量的CNP或单独的H4抗体在递送系统中系统地或口服地施用以遏制与循环染色质片段的负担增加相关的病理状况。