Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof
    1.
    发明授权
    Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof 失效
    重组微生物3-羟基丁酸脱氢酶一种生产方法及其应用

    公开(公告)号:US06255093B1

    公开(公告)日:2001-07-03

    申请号:US09287097

    申请日:1999-04-07

    IPC分类号: C12N904

    CPC分类号: C12N9/0006

    摘要: DNA molecule comprising a structural gene which codes for a bacterial protein with the enzymatic activity of 3-hydroxybutyrate dehydrogenase (3-HBDH), a recombinant DNA containing the DNA molecule, microorganisms transformed accordingly, a process for obtaining 3-HBDH by culturing a suitable transformed microorganism as well as a method for the determination of ketone bodies in the presence of the enzyme. A structural gene from Rhodobacter sphaeroides is preferred.

    摘要翻译: 包含编码具有3-羟基丁酸脱氢酶(3-HBDH)的酶活性的细菌蛋白质的结构基因的DNA分子,含有该DNA分子的重组DNA,相应地转化的微生物,通过培养合适的方法获得3-HBDH的方法 转化的微生物以及在酶存在下测定酮体的方法。 来自球形红细菌的结构基因是优选的。