公开(公告)号：US07348153B2

公开(公告)日：2008-03-25

申请号：US11286917

申请日：2005-11-23

申请人： Soldano Ferrone ,  Constantin Ioannides ,  James L. Murray ,  Kouichiro Kawano

发明人： Soldano Ferrone ,  Constantin Ioannides ,  James L. Murray ,  Kouichiro Kawano

IPC分类号： G01N33/53

CPC分类号： G01N33/686 ,  G01N33/6863 ,  G01N33/6878

摘要： The invention provides a method for identifying peptides for use in increasing a cytotoxic T lymphocytes (CTL) response to an antigen. The method comprises the steps of comparing the amino acid sequence of the VH and/or VL portions of an anti-idiotypic monoclonal antibody to the amino acid sequence of an antigen to identify regions of homology between the Ab2 and the antigen, and to further identify an HLA binding motif in a homologous region. The identified homologous region which comprises an HLA binding motif defines a peptide sequence that is useful for stimulating a CTL response. Also provided are peptides identified by the method, and a method of using the peptides to stimulate a CTL response in an individual.

摘要翻译： 本发明提供了一种用于鉴定用于增加对抗原的细胞毒性T淋巴细胞（CTL）应答的肽的方法。 该方法包括将抗独特型单克隆抗体的VH部分和/或VL部分的氨基酸序列与抗原的氨基酸序列进行比较的步骤，以鉴定Ab2与抗原之间的同源性区域，并进一步鉴定 同源区的HLA结合基序。 鉴定的包含HLA结合基序的同源区定义了可用于刺激CTL应答的肽序列。 还提供了通过该方法鉴定的肽以及使用肽刺激个体中CTL应答的方法。

公开(公告)号：US20060141532A1

公开(公告)日：2006-06-29

申请号：US11286917

申请日：2005-11-23

申请人： Soldano Ferrone ,  Constantin Ioannides ,  James Murray ,  Kouichiro Kawano

发明人： Soldano Ferrone ,  Constantin Ioannides ,  James Murray ,  Kouichiro Kawano

IPC分类号： G01N33/53

CPC分类号： G01N33/686 ,  G01N33/6863 ,  G01N33/6878

摘要： The invention provides a method for identifying peptides for use in increasing a cytotoxic T lymphocytes (CTL) response to an antigen. The method comprises the steps of comparing the amino acid sequence of the VH and/or VL portions of an anti-idiotypic monoclonal antibody to the amino acid sequence of an antigen to identify regions of homology between the Ab2 and the antigen, and to further identify an HLA binding motif in a homologous region. The identified homologous region which comprises an HLA binding motif defines a peptide sequence that is useful for stimulating a CTL response. Also provided are peptides identified by the method, and a method of using the peptides to stimulate a CTL response in an individual.

摘要翻译： 本发明提供了一种用于鉴定用于增加对抗原的细胞毒性T淋巴细胞（CTL）应答的肽的方法。 该方法包括将抗独特型单克隆抗体的VH部分和/或VL部分的氨基酸序列与抗原的氨基酸序列进行比较的步骤，以鉴定Ab2与抗原之间的同源性区域，并进一步鉴定 同源区的HLA结合基序。 鉴定的包含HLA结合基序的同源区定义了可用于刺激CTL应答的肽序列。 还提供了通过该方法鉴定的肽以及使用肽刺激个体中CTL应答的方法。