公开(公告)号：US08242242B2

公开(公告)日：2012-08-14

申请号：US12444842

申请日：2006-12-15

Applicant: Sun-Chang Kim ,  Ju-Young Lee ,  Bong-Hyun Sung ,  Jun-Hyoung Lee ,  Sang-Hee Lee ,  Kui-Hyeon Kang

Inventor： Sun-Chang Kim ,  Ju-Young Lee ,  Bong-Hyun Sung ,  Jun-Hyoung Lee ,  Sang-Hee Lee ,  Kui-Hyeon Kang

IPC: C12N1/21

CPC classification number: C07K14/4702 ,  C07K2319/81

Abstract: The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an E. coli, wherein the transcription factor comprising zinc finger proteins and transcription factors of prokaryote, and to be engineered E. coli using the same. Specifically, the artificial transcription factors comprising zinc finger domains and transcription factors in E. coli as effector domains are prepared and said artificial transcription library is introduced to E. coli to effectively and artificially regulate gene expression regardless of an activity of endogenous transcription factors in the E. coli and to induce E. coli having various desired phenotypes. Thus, only E. coli having the desired phenotypes useful for industries can be selected and used.

Abstract translation: 本发明涉及人工调节大肠杆菌基因表达的人工转录因子，其中包含锌指蛋白的转录因子和原核生物的转录因子，以及使用其的工程化大肠杆菌。 具体地说，制备了包含锌指结构域和转录因子在大肠杆菌中作为效应子结构域的人工转录因子，并且将所述人工转录文库导入大肠杆菌以有效和人工调节基因表达，而不管内源性转录因子的活性如何 并诱导具有各种所需表型的大肠杆菌。 因此，可以选择和使用仅具有用于工业的期望表型的大肠杆菌。

公开(公告)号：US20090305421A1

公开(公告)日：2009-12-10

申请号：US12291007

申请日：2008-11-04

Applicant: Sun Chang Kim ,  Kui Hyeon Kang ,  Byung Jo Yu ,  Jun Hyoung Lee ,  Bong Hyun Sung ,  Choong Hoon Lee ,  Sang Hee Lee ,  Ju Young Lee ,  Myung Keun Park

Inventor： Sun Chang Kim ,  Kui Hyeon Kang ,  Byung Jo Yu ,  Jun Hyoung Lee ,  Bong Hyun Sung ,  Choong Hoon Lee ,  Sang Hee Lee ,  Ju Young Lee ,  Myung Keun Park

IPC: C12N15/70 ,  C12N1/21

CPC classification number: C12N15/102 ,  C12N15/70 ,  C12N15/902 ,  C12N2800/80 ,  C12N2830/002

Abstract: Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (λ)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method.

Abstract translation: 本文公开了用于缺失特异性染色体区域的重组载体和使用其的缺失目标微生物染色体区域的方法。 具体地，重组载体包含阿拉伯糖诱导型启动子; 编码涉及λ（λ） - 重组的蛋白质的基因; 鼠李糖诱导型启动子; 和编码I-SceI内切核酸酶的基因。 与常规方法相比，本发明能够方便，快速和无标记地连续删除微生物的特定基因。

公开(公告)号：US20100136663A1

公开(公告)日：2010-06-03

申请号：US12444842

申请日：2006-12-15

Applicant: Sun-Chang KIM ,  Ju-Young Lee ,  Bong-Hyun Sung ,  Jun-Hyoung Lee ,  Sang-Hee Lee ,  Kui-Hyeon Kang

Inventor： Sun-Chang KIM ,  Ju-Young Lee ,  Bong-Hyun Sung ,  Jun-Hyoung Lee ,  Sang-Hee Lee ,  Kui-Hyeon Kang

IPC: C12N1/21 ,  C07K14/435

CPC classification number: C07K14/4702 ,  C07K2319/81

Abstract: The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an E. coli, wherein the transcription factor comprising zinc finger proteins and transcription factors of prokaryote, and to be engineered E. coli using the same. Specifically, the artificial transcription factors in E. coli as effector domains are prepared and said artificial transcription library is introduced to E. coli to effectively and artificially regulate gene expression regardless of an activity of endogenous transcription factors in the E. coli and to induce E. coli having various desired phenotypes. Thus, only E. coli having the desired phenotypes useful for industries can be selected and used.

Abstract translation: 本发明涉及人工调节大肠杆菌基因表达的人工转录因子，其中包含锌指蛋白的转录因子和原核生物的转录因子，以及使用其的工程化大肠杆菌。 具体而言，制备大肠杆菌作为效应域的人工转录因子，并且将所述人工转录文库导入大肠杆菌以有效和人为地调节基因表达，而不管大肠杆菌内源性转录因子的活性如何，并诱导E 具有各种所需表型的大肠杆菌。 因此，可以选择和使用仅具有用于工业的期望表型的大肠杆菌。