公开(公告)号：US09290787B2

公开(公告)日：2016-03-22

申请号：US14119149

申请日：2012-05-15

Applicant: Xinde Xu ,  Mingqing Jiao ,  Dong Shao ,  Bin Shao ,  Leiming Yu

Inventor： Xinde Xu ,  Mingqing Jiao ,  Dong Shao ,  Bin Shao ,  Leiming Yu

IPC: C07C1/00 ,  C12P23/00 ,  A23L1/303 ,  A23L1/275 ,  A61K31/015

CPC classification number: C12P23/00 ,  A23L5/44 ,  A23L33/155 ,  A23V2002/00 ,  A61K31/015 ,  A23V2250/702

Abstract: The present invention provides a method for producing and purifying β-carotene by Blakeslea trispora fermentation and use thereof. The method comprises the following steps: a) separately inoculating the Blakeslea trispora strains onto a PDA culture medium so as to obtain a spore suspension; b) propagating spores in a seeding tank so as to obtain seeds for fermentation; c) inoculating the seeds for fermentation onto a fermenter and fermenting said seeds; d) adjusting the fermentation liquid to be basic by using an organic or inorganic base, and filtering so as to obtain wet mycelia; e) treating the wet mycelia with a hydrophobic non-polar organic solvent; f) mixing the wet mycelia with an organic solvent of ester and obtaining a concentrated solution by extracting; g) adding a saturated monohydric alcohol into the concentrated solution, and filtering and crystallizing so as to obtain pure ss-carotene. The content of the ss-carotene in the present invention exceeds 96%, and the yield is above 85%.

Abstract translation: 本发明提供了通过三孢a菌发酵及其应用来生产和提纯β-胡萝卜素的方法。 该方法包括以下步骤：a）将单孢菌菌株分别接种到PDA培养基上，得到孢子悬浮液; b）在播种槽中繁殖孢子，以获得用于发酵的种子; c）将种子接种发酵到发酵罐上并发酵所述种子; d）通过使用有机或无机碱调节发酵液为碱性，过滤以获得湿菌丝体; e）用疏水性非极性有机溶剂处理湿菌丝体; f）将湿菌丝体与酯的有机溶剂混合，通过萃取获得浓缩溶液; g）向浓缩溶液中加入饱和一元醇，过滤结晶，得到纯β-胡萝卜素。 本发明中β-胡萝卜素的含量超过96％，收率高于85％。

公开(公告)号：US20140187829A1

公开(公告)日：2014-07-03

申请号：US14119149

申请日：2012-05-15

Applicant: Xinde Xu ,  Mingqing Jiao ,  Dong Shao ,  Bin Shao ,  Leiming Yu

Inventor： Xinde Xu ,  Mingqing Jiao ,  Dong Shao ,  Bin Shao ,  Leiming Yu

IPC: C12P23/00

CPC classification number: C12P23/00 ,  A23L5/44 ,  A23L33/155 ,  A23V2002/00 ,  A61K31/015 ,  A23V2250/702

Abstract: The present invention provides a method for producing and purifying β-carotene by Blakeslea trispora fermentation and use thereof. The method comprises the following steps: a) separately inoculating the Blakeslea trispora strains onto a PDA culture medium so as to obtain a spore suspension; b) propagating spores in a seeding tank so as to obtain seeds for fermentation; c) inoculating the seeds for fermentation onto a fermenter and fermenting said seeds; d) adjusting the fermentation liquid to be basic by using an organic or inorganic base, and filtering so as to obtain wet mycelia; e) treating the wet mycelia with a hydrophobic non-polar organic solvent; f) mixing the wet mycelia with an organic solvent of ester and obtaining a concentrated solution by extracting; g) adding a saturated monohydric alcohol into the concentrated solution, and filtering and crystallizing so as to obtain pure ss-carotene. The content of the ss-carotene in the present invention exceeds 96%, and the yield is above 85%.

Abstract translation: 本发明提供了通过三孢a菌发酵及其应用来生产和提纯β-胡萝卜素的方法。 该方法包括以下步骤：a）将单孢菌菌株分别接种到PDA培养基上，得到孢子悬浮液; b）在播种槽中繁殖孢子，以获得用于发酵的种子; c）将种子接种发酵到发酵罐上并发酵所述种子; d）通过使用有机或无机碱调节发酵液为碱性，过滤以获得湿菌丝体; e）用疏水性非极性有机溶剂处理湿菌丝体; f）将湿菌丝体与酯的有机溶剂混合，通过萃取获得浓缩溶液; g）向浓缩溶液中加入饱和一元醇，过滤结晶，得到纯β-胡萝卜素。 本发明中β-胡萝卜素的含量超过96％，收率高于85％。